Production of Japanese encephalitis virus-like particles in insect cells
Production of Japanese encephalitis virus-like particles in insect cells
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DOI：
10.4161/bioe.24514
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发表时间：
2013-05
期刊：
影响因子：
4.9
通讯作者：
H. Yamaji;E. Konishi
中科院分区：
文献类型：
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作者：
H. Yamaji;E. Konishi
病毒样颗粒 (VLP) 由一种或多种重组病毒表面蛋白组成，它们自发组装成颗粒结构，而不掺入病毒 DNA 或 RNA。杆状病毒-昆虫细胞系统已广泛用于生产包括VLP在内的重组病毒蛋白。虽然杆状病毒-昆虫细胞系统指导重组蛋白在分批培养中瞬时表达，但稳定转化的昆虫细胞允许组成型生产。在我们最近的研究中，日本脑炎 (JE) VLP 的分泌型由 Trichoplusia ni BTI-TN-5B1-4 (High Five) 细胞成功产生，该细胞被设计为共表达乙脑病毒 (JEV) 前膜 (prM) 和包膜 (E) 蛋白。与杆状病毒-昆虫细胞系统相比，重组 High Five 细胞获得了更高的 E 蛋白产量。这项研究表明，重组昆虫细胞为高水平生产用作疫苗和诊断抗原的 VLP 提供了一种有前途的方法。
Virus-like particles (VLPs) are composed of one or several recombinant viral surface proteins that spontaneously assemble into particulate structures without the incorporation of virus DNA or RNA. The baculovirus-insect cell system has been used extensively for the production of recombinant virus proteins including VLPs. While the baculovirus-insect cell system directs the transient expression of recombinant proteins in a batch culture, stably transformed insect cells allow constitutive production. In our recent study, a secretory form of Japanese encephalitis (JE) VLPs was successfully produced by Trichoplusia ni BTI-TN-5B1-4 (High Five) cells engineered to coexpress the JE virus (JEV) premembrane (prM) and envelope (E) proteins. A higher yield of E protein was attained with recombinant High Five cells than with the baculovirus-insect cell system. This study demonstrated that recombinant insect cells offer a promising approach to the high-level production of VLPs for use as vaccines and diagnostic antigens.