Semiconductor nanocrystals as fluorescent biological labels
Semiconductor nanocrystals as fluorescent biological labels
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DOI：
10.1126/science.281.5385.2013
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发表时间：
1998-09-25
期刊：
影响因子：
56.9
通讯作者：
Alivisatos, AP
中科院分区：
文献类型：
--
作者：
Bruchez, M;Moronne, M;Alivisatos, AP
制备了半导体纳米晶体，用作生物染色和诊断中的荧光探针。与传统的荧光团相比，纳米晶体具有窄的，可调谐的。对称发射光谱且光化学稳定。在小鼠成纤维细胞上的双发射、单激发标记实验中证明了宽的、连续的激发光谱的优点。因此，这些荧光探针是互补的，并且在某些情况下可能上级现有的荧光团。
Semiconductor nanocrystals were prepared for use as fluorescent probes in biological staining and diagnostics. Compared with conventional fluorophores, the nanocrystals have a narrow, tunable. symmetric emission spectrum and are photochemically stable. The advantages of the broad, continuous excitation spectrum were demonstrated in a dual-emission, single-excitation labeling experiment on mouse fibroblasts. These nanocrystal probes are thus complementary and in some cases may be superior to existing fluorophores.