Serum antibodies to enterohepatic Helicobacter spp. in patients with chronic liver diseases and in a population with high prevalence of H-pylori infection
Serum antibodies to enterohepatic Helicobacter spp. in patients with chronic liver diseases and in a population with high prevalence of H-pylori infection
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DOI：
10.1016/j.dld.2005.12.008
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发表时间：
2006-03-01
影响因子：
4.5
通讯作者：
Uibo, R
中科院分区：
文献类型：
--
作者：
Vorobjova, T;Nilsson, I;Uibo, R
背景肠道螺杆菌可能是慢性肝胆疾病的危险因素。分析各种胆道和慢性肝病患者血清中三种肠道螺杆菌的抗体水平，并将结果与已知幽门螺杆菌感染率高的成年人群和健康献血员的相应参数进行比较，以探讨肠道螺杆菌与慢性肝病的可能相关性。分析了90例各种慢性肝病患者、121例爱沙尼亚成年人和68例献血员的血清。血清，以前测试H。pylori中分析抗肝螺杆菌、胆汁螺杆菌和鸡白痢螺杆菌的IgG。ELISA最初用于筛查和排除阴性病例。ELISA阳性的血清用免疫印迹法进一步分析。去除H. pylori和肠道菌，血清用裂解的H. pylori细胞。肝脏疾病患者的H. hepaticus和H. bilis与成年人群组比较（p分别为0.0001和0.04），与H.与献血员相比，肝细胞癌的发生率（p = 0.01）。自身免疫性肝炎患者对肠道螺杆菌无明显抗体反应。与其他慢性肝病患者相比。除自身免疫性肝炎患者外，慢性肝病患者的抗H抗体水平均升高。bilis/H.与人群和献血员相比，肝内幽门螺杆菌感染表明肠道螺杆菌在慢性肝病的自然病程中可能发挥作用。免疫印迹法似乎是一种很有前途的方法，这些挑剔的病原体感染的血清学诊断。(C)2005年意大利胃肠病学编辑由爱思唯尔有限公司出版。保留所有权利。
Background. Enteric Helicobacter species might be a risk factor for chronic liver and biliary tract diseases.Aims. To analyse serum antibody levels to three enteric Helicobacter species in patients with various biliary tract and chronic liver diseases and compare results with corresponding parameters for an adult population group, known to have a high prevalence of Helicobacter pylori infection, and with healthy blood donors, to explore a possible association of enteric Helicobacter with chronic liver diseases.Subjects. Sera of 90 patients with various chronic liver diseases, 121 Estonian adult persons and 68 blood donors were analysed.Methods. Sera, previously tested for H. pylori were analysed for IgG to Helicobacter hepaticus, Helicobacter bilis and Helicobacter pullorum. ELISA was initially used for screening and exclusion of negative cases. Sera with positive ELISA results were further analysed by immunoblot. To remove cross-reactive antibodies between H. pylori and the enteric species, sera were pre-absorbed with lysed H. pylori cells.Results. Liver patients showed a significantly higher seroprevalence to H. hepaticus and H. bilis, compared with the adult population group (p = 0.0001 and 0.04, respectively), and to H. hepaticus, compared with blood donors (p = 0.01). Patients with autoimmune hepatitis showed no significant antibody reactivity to the enteric Helicobacter spp. in contrast to patients with other chronic liver diseases.Conclusion. Patients with chronic liver diseases, except autoimmune hepatitis patients, showed increased antibody levels to H. bilis/H. hepaticus compared with the population and blood donors indicating a possible role of enteric Helicobacter in the natural course of chronic liver diseases. Immunoblot seems to be a promising method for serodiagnosis of infections with these fastidious pathogens. (C) 2005 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.