MODULATION OF PERIPHERAL BENZODIAZEPINE RECEPTORS IN FEMALE RAT GENITAL ORGANS BY VARIOUS GONADAL-STEROIDS
MODULATION OF PERIPHERAL BENZODIAZEPINE RECEPTORS IN FEMALE RAT GENITAL ORGANS BY VARIOUS GONADAL-STEROIDS
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DOI：
10.1016/0024-3205(94)90131-7
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发表时间：
1994-01-01
期刊：
影响因子：
6.1
通讯作者：
GAVISH, M
中科院分区：
文献类型：
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作者：
BARAMI, S;AMIRI, Z;GAVISH, M
在短期和长期的睾酮（T），孕酮（P-4）和己烯雌酚（DES）治疗下，研究了未成年大鼠卵巢，输卵管，子宫和肾脏外周苯二氮卓受体（PBR）。与完整大鼠相比，在卵巢（1.6倍）、输卵管（2.0倍）和子宫（1.4倍）中用T处理4天后观察到PBR特异性结合显著增加。用P-4处理4天可增加卵巢中的PBR特异性结合（1.5倍），但在输卵管或子宫中未检测到变化。与此相反，PBR特异性结合显着减少了10天的治疗与T或P-4：40和12%，分别在卵巢和35和40%，分别在输卵管。用T处理10天使子宫中的PBR特异性结合减少25%，但用P-4处理相同时间间隔并不改变子宫中的特异性结合。用DES处理4或10天显著增加卵巢（1.5倍）、输卵管（2.4倍）和子宫（1.9倍）中的PBR特异性结合。Scatchard分析表明，PBR特异性结合的变化是由于PBR密度值而不是PBR亲和力值的变化。在任何这些处理后，在肾脏中未发现PBR特异性结合的变化。两者合计，这表明，卵巢中的PBR密度改变了外源性给药的类固醇，通常是在卵巢中生物合成。此外，输卵管和子宫中的PBR密度通过所用的各种类固醇而改变，这可能意味着卵巢类固醇生成中发生的变化应影响这些器官中的PBR密度。
Peripheral benzodiazepine receptors (PBR) in the ovary, oviduct, uterus, and kidney of immature rats were studied under short- and long-term treatment with testosterone (T), progesterone (P-4), and diethylstilbestrol (DES). A significant increase in PBR specific binding was observed after 4 days' treatment with T in the ovary (1.6-fold), oviduct (2.0-fold), and uterus (1.4-fold) compared with intact rats. Four days' treatment with P-4 increased PBR specific binding in the ovary (1.5-fold), but no changes were detected in the oviduct or uterus. In contrast, PBR specific binding was significantly reduced by 10 days' treatment with T or P-4: 40 and 12%, respectively, in the ovary and 35 and 40%, respectively, in the oviduct. Ten days' treatment with T reduced PBR specific binding in the uterus by 25%, but the same interval of treatment with P-4 did not alter specific binding in the uterus. Four or 10 days' treatment with DES significantly increased PBR specific binding in the ovary (1.5-fold), oviduct (2.4-fold), and uterus (1.9-fold). Scatchard analysis revealed that the changes in the PBR specific binding were due to a change in PBR density values rather than PBR affinity values. No change in PBR specific binding was found in the kidney following any of these treatments. Taken together, it is suggested that PBR density in the ovary is altered by exogenously administered steroids that usually are biosynthesized in the ovary. Additionally, the altered PBR density in the oviduct and uterus via the various steroids employed may imply that changes occurring in ovarian steroidogenesis should affect PBR density in these organs.