Swelling and mechanical properties of biopolymer hydrogels containing chitosan and bovine serum albumin
Swelling and mechanical properties of biopolymer hydrogels containing chitosan and bovine serum albumin
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DOI：
10.1021/bm060133y
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发表时间：
2006-11-13
影响因子：
6.2
通讯作者：
Adams, Sarah
中科院分区：
文献类型：
--
作者：
Butler, Michael F.;Clark, Allan H.;Adams, Sarah
对壳聚糖、牛血清白蛋白（BSA）、壳聚糖/BSA混合物与京尼平交联形成的水凝胶的溶胀和力学性能进行了实验和理论研究。交联壳聚糖水凝胶的性质解释在其溶胀行为，这导致在低于pK值的溶胀比逐渐增加，但由此其溶胀能力被消除的存在下，筛选的电荷的盐。然而，溶胀比的理论和实验计算的比较表明，从浪费的交联，聚合京尼平交联的形成所产生的并发症必须考虑之前，可以实现定量预测。交联的BSA水凝胶即使在盐的存在下也会溶胀，并且在pH = 3以下观察到溶胀的显著增加，这被解释为导致分子展开的蛋白质的酸诱导变性的结果。然而，Swedish BSA水凝胶的机械强度较弱。由壳聚糖和BSA的交联混合物制成的复合凝胶表现出BSA的溶胀行为与壳聚糖的机械性能相结合，因此被认为最适合用于胃环境。
Experimental and theoretical investigations of the swelling and mechanical properties of hydrogels formed from chitosan, bovine serum albumin (BSA), and chitosan/BSA mixtures cross-linked with genipin were performed. The properties of cross-linked chitosan hydrogels were explained in terms of its polyelectrolyte behavior, which led to a gradual increase in swelling ratio below the pK value, but whereby its swelling ability was eliminated by the presence of salt that screened the charges. Comparison of theoretical and experimental calculations of the swelling ratio, however, indicated that complications arising from wastage of cross-links, and formation of polymerized genipin cross-links must be considered before quantitative prediction can be achieved. Cross-linked BSA hydrogels swelled even in the presence of salt, and a marked increase in swelling was observed below pH = 3 that was explained as the result of an acid induced denaturation of the protein that led to unfolding of the molecule. Swollen BSA hydrogels were mechanically weak, however. Composite gels made from a cross-linked mixture of chitosan and BSA exhibited the swelling behavior of BSA combined with the mechanical properties of chitosan and were therefore considered most suitable for use in a gastric environment.