IMMUNOPROPHYLAXIS AND IMMUNOTHERAPY OF RESPIRATORY SYNCYTIAL VIRUS-INFECTED MICE WITH RESPIRATORY SYNCYTIAL VIRUS-SPECIFIC IMMUNE SERUM
IMMUNOPROPHYLAXIS AND IMMUNOTHERAPY OF RESPIRATORY SYNCYTIAL VIRUS-INFECTED MICE WITH RESPIRATORY SYNCYTIAL VIRUS-SPECIFIC IMMUNE SERUM
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DOI：
10.1203/00006450-199308000-00013
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发表时间：
1993-08-01
影响因子：
3.6
通讯作者：
GRUBER, WC
中科院分区：
文献类型：
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作者：
GRAHAM, BS;DAVIS, TH;GRUBER, WC
在BALB/c小鼠呼吸道合胞病毒（RSV）感染的疾病模型上，观察了被动RSV免疫血清在接种前、接种后和接种后第5天的预防和治疗作用。接种前和接种后预防可减少原发性RSV感染后肺部RSV复制，预防疾病。第5天处理不影响肺中RSV的峰值滴度，但导致比未处理小鼠更快地从疾病中恢复。预防性治疗可预防原发性RSV感染后肺中的抗体应答和淋巴细胞浸润。治疗引起抗体减少和病理反应。尽管再感染后的病情较轻，但预防措施增加了再感染的易感性。接受治疗的小鼠对再感染的敏感性低于接受免疫治疗的小鼠，但它们在再激发后也发生了轻度疾病。RSV感染的被动抗体预防和治疗是减轻原发性RSV感染引起的下呼吸道疾病的有希望的方法。测量疾病终点和病理学的能力使得RSV感染的BALB/c小鼠模型成为用于免疫预防和免疫治疗方式的临床前评价的有用系统。
The effects of passive respiratory syncytial virus (RSV) immune serum given as preinoculation prophylaxis, postinoculation prophylaxis, and as therapy on d 5 after inoculation were evaluated in an illness model of RSV infection in BALB/c mice. Pre- and postinoculation prophylaxis reduced RSV replication in lung after primary RSV infection and prevented illness. Day 5 treatment did not affect peak titer of RSV in lung but resulted in more rapid recovery from illness than seen in untreated mice. Prophylaxis prevented antibody responses and lymphocytic infiltrates in lung after primary RSV infection. Treatment caused diminished antibody and pathologic responses. Prophylaxis increased susceptibility to reinfection, although illness after rechallenge was mild. Mice treated therapeutically were less susceptible to reinfection than mice treated prophylactically, but they also experienced mild illness after rechallenge. Passive antibody prophylaxis and treatment of RSV infection are promising approaches to attenuating lower respiratory tract illness from primary RSV infection. The ability to measure illness endpoints and pathology make the BALB/c mouse model of RSV infection a useful system for the preclinical evaluation of immunoprophylactic and immunotherapeutic modalities.