Long-term three-dimensional neural tissue cultures in functionalized self-assembling peptide hydrogels, Matrigel and Collagen I
Long-term three-dimensional neural tissue cultures in functionalized self-assembling peptide hydrogels, Matrigel and Collagen I
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DOI：
10.1016/j.actbio.2012.09.010
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发表时间：
2013-02-01
影响因子：
9.7
通讯作者：
Zhang, Shuguang
中科院分区：
文献类型：
--
作者：
Koutsopoulos, Sotirios;Zhang, Shuguang
具有自组装特性的设计肽形成纳米纤维，其进一步组织形成由高达99.5%的水组成的水凝胶。我们在这里提出了神经干细胞到肽水凝胶支架的封装。这导致三维（3-D）神经组织培养物，其中当在无血清培养基中培养时，神经干细胞分化成祖神经细胞、神经元、星形胶质细胞和少突胶质细胞。细胞存活研究表明，肽水凝胶中的神经细胞至少能存活5个月。相比之下，包封在胶原蛋白I中的神经干细胞分化较差，并且没有显著迁移，因此形成簇。我们发现，对于1-2周的培养期，神经干细胞在Matrigel中增殖和分化更好。然而，在长期研究中，Matrigel中的细胞群体减少，而在肽水凝胶中的神经组织培养物中观察到更好的细胞存活率。与在未修饰的肽水凝胶中培养神经细胞时观察到的那些相比，具有细胞粘附和细胞分化基序的肽官能化显示出上级细胞存活和分化特性。这些设计的3-D工程组织培养系统具有作为组织再生的组织替代物的潜在用途。肽水凝胶的明确的化学和物理性质以及无血清培养基的使用允许在仿生3-D环境中对神经细胞进行更真实的生物学研究。(C)2012 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Designer peptides with self-assembling properties form nanofibers which are further organized to form a hydrogel consisting of up to 99.5% water. We present here the encapsulation of neural stem cells into peptide nanofiber hydrogel scaffolds. This results in three-dimensional (3-D) neural tissue cultures in which neural stem cells differentiate into progenitor neural cells, neurons, astrocytes and oligodendrocytes when cultured in serum-free medium. Cell survival studies showed that neural cells in peptide hydrogels thrive for at least 5 months. In contrast, neural stem cells encapsulated in Collagen I were poorly differentiated and did not migrate significantly, thus forming clusters. We show that for culture periods of 1-2 weeks, neural stem cells proliferate and differentiate better in Matrigel. However, in long-term studies, the population of cells in Matrigel decreases whereas better cell survival rates are observed in neural tissue cultures in peptide hydrogels. Peptide functionalization with cell adhesion and cell differentiation motifs show superior cell survival and differentiation properties compared to those observed upon culturing neural cells in non-modified peptide hydrogels. These designed 3-D engineered tissue culturing systems have a potential use as tissue surrogates for tissue regeneration. The well-defined chemical and physical properties of the peptide nanofiber hydrogels and the use of serum-free medium allow for more realistic biological studies of neural cells in a biomimetic 3-D environment. (C) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.