The effect of calcium phosphate microstructure on bone-related cells in vitro
The effect of calcium phosphate microstructure on bone-related cells in vitro
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DOI：
10.1016/j.biomaterials.2008.04.039
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发表时间：
2008-08-01
期刊：
影响因子：
14
通讯作者：
Watari, Fumio
中科院分区：
文献类型：
--
作者：
Li, Xiaoming;van Blitterswijk, Clemens A.;Watari, Fumio
软组织植入后，微结构对于磷酸钙材料的诱导骨形成至关重要。我们特此评估了在三种磷酸钙陶瓷盘上培养的三种类型细胞的活性（细胞附着、增殖、ALP/DNA 和蛋白质/DNA），以研究微观结构如何在诱导骨形成中发挥作用。三种具有相同化学成分和相同圆分割尺寸 10.0 x 1.0 mm(3) 的双相磷酸钙 (BCP) 陶瓷盘（BCP1150-P、BCP1150-D 和 BCP1300），或者具有相似的微孔尺寸和表面粗糙度但不同的表面积（BCP1150-P 与 BCP1150-D）或具有相似的表面积但不同的微孔尺寸和不同的粗糙度（BCP1150-D 与 BCP1300），已准备好。在 BCP 圆盘上常规培养 C2C12、人骨髓基质细胞 (HBMSC) 和 MC3T3-E1 细胞表明，表面粗糙度不影响所评估的所有细胞类型的细胞附着、细胞增殖和 ALP 表达，而表面积确实影响细胞功能。 BCP1150-P上的C2C12的ALP/DNA具有较大的表面积，显着高于BCP1300和BCP1150-D。此外，在预先浸泡在含有额外rhBMP-2的培养基中的所有三种BCP上培养的所有细胞均具有比常规细胞培养物更高的ALP表达。与BCP1300和BCP1150-D相比，将光盘预浸泡在含有rhBMP-2的培养基中后，BCP1150-P上所有测试细胞的ALP/DNA增加更多。结果表明，增加表面积，微结构磷酸钙材料可能会集中更多的蛋白质（包括骨诱导蛋白），从而将诱导细胞分化为形成诱导骨的成骨细胞。 (c) 2008 Elsevier Ltd. 保留所有权利。
Microstructure is essential for inductive bone formation in calcium phosphate materials after soft tissue implantation. We hereby evaluated activities (cell attachment, proliferation, ALP/DNA and protein/DNA) of three types of cells cultured on three kinds of calcium phosphate ceramic discs to study how microstructure takes its role in inductive bone formation. Three kinds of biphasic calcium phosphate (BCP) ceramic discs with the same chemistry and the same dimension of circle divide 10.0 x 1.0 mm(3) (BCP1150-P, BCP1150-D and BCP1300), either having similar micropore sizes and surface roughness but different surface area (BCP1150-P vs BCP1150-D) or having similar surface area but different micropore sizes and different roughness (BCP1150-D vs BCP1300), were prepared. Conventionally Culturing C2C12, human bone marrow stromal cells (HBMSC) and MC3T3-E1 cells on BCP discs showed that, surface roughness did not affect cell attachment, cell proliferation and ALP expression of all cell types evaluated, while surface area did affect cell functions. ALP/DNA of C2C12 on BCP1150-P, having larger surface area, was significantly higher than on BCP1300 and BCP1150-D. Furthermore, all cells cultured on all of the three kinds of BCPs pre-soaked in culture medium having additional rhBMP-2 had a higher ALP expression than the conventional cell culture. Comparing with on BCP1300 and BCP1150-D, ALP/DNA of all cells tested increased more on BCP1150-P after the discs were pre-soaked in culture medium with rhBMP-2. The results indicated that increasing surface areas, microstructured calcium phosphate materials might concentrate more proteins (including bone-inducing proteins) that differentiate inducible cells to osteogenic cells that form inductive bone. (c) 2008 Elsevier Ltd. All rights reserved.