Modeling mechanosensing and its effect on the migration and proliferation of adherent cells
Modeling mechanosensing and its effect on the migration and proliferation of adherent cells
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DOI：
10.1016/j.actbio.2007.10.014
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发表时间：
2008-05-01
影响因子：
9.7
通讯作者：
Doblare, Manuel
中科院分区：
文献类型：
--
作者：
Moreo, Pedro;Garcia-Aznar, Jose Manuel;Doblare, Manuel
正常贴壁细胞的行为受到它们所锚定的底物的刚度的影响。细胞能够通过主动产生收缩力来检测基质的机械特性，并利用这些信息进行迁移和增殖。特别是，细胞爬行的速度和方向以及细胞增殖的速度随底物顺应性和预应变而变化。在这项工作中，我们提出了一个基于骨骼肌行为的经典Hill模型扩展的主动机械传感模型。我们还提出了一种热力学方法来模拟由机械刺激调节的细胞迁移，并提出了一种依赖于机械环境的增殖理论。这些贡献产生了一个概念上简单的数学公式，具有直接和廉价的计算实现，产生与许多实验一致的结果。该模型可以在生物和医学的实际应用中成为一个有用的工具，在细胞-底物相互作用以及底物力学行为起重要作用的情况下，例如组织工程应用的设计。(c) 2007材料学报Elsevier Ltd.出版。版权所有。
The behavior of normal adherent cells is influenced by the stiffness of the substrate they are anchored to. Cells are able to detect substrate mechanical properties by actively generating contractile forces and use this information to migrate and proliferate. In particular, the speed and direction of cell crawling, as well as the rate of cell proliferation, vary with the substrate compliance and prestrain. In this work, we present an active mechanosensing model based on an extension of the classical Hill's model for skeletal muscle behavior. We also propose a thermodynamical approach to model cell migration regulated by mechanical stimuli and a proliferation theory also depending on the mechanical environment. These contributions give rise to a conceptually simple mathematical formulation with a straightforward and inexpensive computational implementation, yielding results consistent with numerous experiments. The model can be a useful tool for practical applications in biology and medicine in situations where cell-substrate interaction as well as substrate mechanical behavior play an important role, such as the design of tissue engineering applications. (c) 2007 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.