Generation and characterization of three induced pluripotent stem cell lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) from a 51-year-old
Generation and characterization of three induced pluripotent stem cell lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) from a 51-year-old
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来自 51 岁患者的三种诱导多能干细胞系（NUIGi046-A、NUIGi046-B、NUIGi046-C）的生成和表征
DOI：
10.1016/j.scr.2021.102607
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发表时间：
2021
影响因子：
1.2
通讯作者：
Sanbing Shen
中科院分区：
文献类型：
--
作者：
Min Liu;Ning Ge;Yongfeng Han;Jamie Reilly;Meimei Yang;Fan Yang;Janusz Krawczyk;Veronica McInerney;Timothy O’Brien;Terence Prendiville;Sanbing Shen
由健康的51岁高加索男性捐献皮肤穿刺活检，并通过使用表达OCT 4、SOX 2、KLF 4和c-MYC的非整合型仙台病毒将真皮成纤维细胞重编程为人诱导多能干细胞（hiPSC）系。三个iPSC系（NUIGi 046-A、NUIGi 046-B、NUIGi 046-C）高度表达多能标记物，并且能够分化成内胚层、中胚层和外胚层来源的细胞。这些iPSC可以作为对照提供，并与基因组编辑和三维（3D）系统相结合。它们可用于人类疾病建模和药物筛选。
Skin punch biopsy was donated by a healthy 51-year-old Caucasian male and the dermal fibroblasts were reprogrammed into human induced pluripotent stem cell (hiPSC) lines by using non-integrative Sendai viruses expressing OCT4, SOX2, KLF4 and c-MYC. Three iPSC lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) highly expressed the pluripotent markers and were capable of differentiating into cells of endodermal, mesodermal, and ectodermal origin. These iPSCs can be offered as controls and in combination with genome-editing and three-dimensional (3D) system. They may be used for human disease modelling and drug screening.