HABENULA AND THALAMUS CELL TRANSPLANTS RESTORE NORMAL SLEEP BEHAVIORS DISRUPTED BY DENERVATION OF THE INTERPEDUNCULAR NUCLEUS
HABENULA AND THALAMUS CELL TRANSPLANTS RESTORE NORMAL SLEEP BEHAVIORS DISRUPTED BY DENERVATION OF THE INTERPEDUNCULAR NUCLEUS
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DOI：
10.1523/jneurosci.12-08-03282.1992
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发表时间：
1992-08-01
影响因子：
5.3
通讯作者：
MURRAY, M
中科院分区：
文献类型：
--
作者：
HAUN, F;ECKENRODE, TC;MURRAY, M
先前的伴随研究（Eckenrode等人，1992）表明，在损伤后，将胎儿缰核细胞置于脚间核（IPN）附近的细胞悬浮移植物恢复了围产期和成年宿主中IPN的缰靶亚核中的P物质（SP）染色的正常模式，并恢复了围产期宿主的IPN中的ChAT染色。同样放置的胚胎丘脑细胞移植只恢复ChAT染色在IPN的成人主机。在这项研究中，我们研究了这些恢复的染色模式的功能意义。我们使用了REM期和非REM期睡眠完整性的行为测量，“花盆”试验，并分析了（1）正常成年大鼠，（2）FR损伤对照动物（新生儿或成年手术），（3）接受FR损伤和胎儿缰核细胞移植的动物（围产期或成年宿主），和（4）接受FR损伤和胎儿丘脑细胞移植的动物（成年宿主）。FR损伤显著减少REM睡眠的肌张力成分，并减少睡眠发作的持续时间。在IPN中恢复SP染色的移植物（将缰移植到围产期或成人病变宿主中）恢复REM弛缓的正常频率;恢复ChAT染色的移植物（将缰移植到围产期宿主中或将丘脑移植到成人宿主中）恢复睡眠发作的正常持续时间。移植物中SP免疫反应细胞的数量预测REM肌张力减退的恢复，而缰（而不是丘脑）移植物中ChAT细胞的数量预测睡眠时间的恢复。我们的结论是，IPN是重要的正常睡眠模式的调节，特别是缰SP目标的发射机特定的神经支配调制REM睡眠的完整性，而缰胆碱能靶的发射机特定的神经支配调制睡眠时间，但只有当胆碱能神经支配是由正常的缰传入介导的。
The preceding companion study (Eckenrode et al., 1992) showed that cell suspension transplants of fetal habenula cells placed near the interpeduncular nucleus (IPN) following lesions of the fasciculus retroflexus (FR) restore the normal pattern of substance P (SP) staining in habenular target subnuclei of the IPN in both perinatal and adult hosts, and restore ChAT staining in the IPN of perinatal hosts. Similarly placed transplants of fetal thalamus cells only restore ChAT staining in the IPN of adult hosts. In this study, we examined the functional significance of these restored staining patterns. We used a behavioral measure of the integrity of REM-stage and non-REM-stage sleep, the "flower pot" test, and assayed (1) normal adult rats, (2) FR-lesioned control animals (neonatal or adult operates), (3) animals receiving FR lesions and transplants of fetal habenula cells (perinatal or adult hosts), and (4) animals receiving FR lesions and transplants of fetal thalamus cells (adult hosts). FR lesions decrease markedly the muscle atonia component of REM sleep and reduce duration of sleep episodes. Transplants that restore SP staining in the IPN (habenular transplants into either perinatal or adult lesion hosts) restore normal frequency of REM atonia; transplants that restore ChAT staining (habenular transplants into perinatal hosts or thalamic transplants into adult hosts) restore normal duration of sleep episodes. The number of SP-immunoreactive cells in the transplants predicts recovery of REM atonia, and the number of ChAT cells in habenular (but not thalamic) transplants predicts restoration of sleep duration. We conclude that the IPN is important in the regulation of normal sleep patterns, and particularly that transmitter-specific innervation of habenular SP targets modulates the integrity of REM sleep, while transmitter-specific innervation of habenular cholinergic targets modulates sleep duration, but only when the cholinergic innervation is mediated by normal habenular afferents.