Evaluation of the antioxidant activity of flavonoids by "ferric reducing antioxidant power" assay and cyclic voltammetry
Evaluation of the antioxidant activity of flavonoids by "ferric reducing antioxidant power" assay and cyclic voltammetry
复制标题
DOI：
10.1016/j.bbagen.2004.11.001
复制
发表时间：
2005-01-18
影响因子：
3
通讯作者：
Saso, L
中科院分区：
文献类型：
--
作者：
Firuzi, O;Lacanna, A;Saso, L
天然存在的酚类化合物黄酮类化合物，最近已被广泛研究其抗氧化性能。黄酮类化合物的结构-抗氧化活性关系（SAR）已针对不同的自由基进行了评估，但“铁还原抗氧化能力”（FRAP）测定，直接确定了化合物的还原能力，并没有用于此目的。在这项研究中，18个不同结构的黄酮类化合物的抗氧化活性进行了评估，FRAP法修改，用于在96孔微孔板。此外，还测量了它们的氧化电位，其在+0.3V（杨梅素）至+1.2V（5-羟基黄酮）的范围内，并且与FRAP测定结果良好一致。槲皮素、非瑟酮和杨梅苷的氧化电位最低，活性最高，分别是Trolox的3.02、2.52和2.28倍。结果表明，B环上的邻二羟基结构和C环上的3-羟基和2，3-双键对抗氧化活性的贡献最大。(C)2004 Elsevier B. V.保留所有权利。
Flavonoids, naturally occurring phenolic compounds, have recently been studied extensively for their antioxidant properties. The structure-antioxidant activity relationships (SAR) of flavonoids have been evaluated against different free radicals, but "ferric reducing antioxidant power" (FRAP) assay, which determines directly the reducing capacity of a compound, has not been used for this purpose. In this study, the antioxidant activities of 18 structurally different flavonoids were evaluated by FRAP assay modified to be used in 96-well microplates. Furthermore, their oxidation potentials were also measured, which were in the range of +0.3 V (myricetin) to +1.2 V (5-hydroxy flavone) and were in good agreement with FRAP assay results. Quercetin, fisetin and myricetin had the lowest oxidation potentials and appeared the most active compounds in FRAP assay and were 3.02, 2.52 and 2.28 times more active than Trolox, respectively. Indications were found that the o-dihydroxy structure in the B ring and the 3-hydroxy group and 2,3-double bond in the C ring give the highest contribution to the antioxidant activity. (C) 2004 Elsevier B.V. All rights reserved.