ASSOCIATION OF CASEIN KINASE-2 WITH NUCLEAR CHROMATIN IN RELATION TO ANDROGENIC REGULATION OF RAT PROSTATE
ASSOCIATION OF CASEIN KINASE-2 WITH NUCLEAR CHROMATIN IN RELATION TO ANDROGENIC REGULATION OF RAT PROSTATE
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DOI：
10.1073/pnas.90.10.4426
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发表时间：
1993-05-15
影响因子：
11.1
通讯作者：
GOUELI, SA
中科院分区：
文献类型：
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作者：
AHMED, K;YENICE, S;GOUELI, SA
酪蛋白激酶2（CK-2）是一种普遍存在的信使非依赖性蛋白丝氨酸/苏氨酸激酶，与生长调控有关。我们研究了CK-2在成年大鼠腹侧前列腺中的活性和亚细胞定位与雄激素戒断和给药的关系。雄激素剥夺去势导致前列腺细胞核CK-2活性比胞浆CK-2活性下降更快。与染色质相关的细胞核CK-2的减少速度甚至比整个细胞核中的减少速度更快。对144小时前去势的成年大鼠单次给予5 α-双氢睾酮，可缓解这些事件，并伴有染色质相关CK-2活性的早期差异性增强，同时伴有细胞溶质中CK-2活性的降低。细胞核CK-2活性的变化与细胞核内的免疫抑制酶蛋白相关。我们认为，雄激素引起CK-2从细胞质易位到细胞核（核质），在那里它与染色质成分的增强关联发生。相反，由于去势导致的循环雄激素的撤回引起CK-2从染色质上解离，并最终使核质CK-2易位到细胞质中。CK-2与核染色质的关联的调节可能代表了核CK-2与前列腺中雄激素作用相关的转录后调节的重要机制。
Casein kinase 2 (CK-2) is a ubiquitous messenger-independent protein serine/threonine kinase that has been implicated in growth control. We have studied the activity and subcellular location of CK-2 in adult rat ventral prostate in relation to androgen withdrawal and administration. Androgen deprivation by castration results in a faster decline in CK-2 activity associated with prostatic nuclei than that in the cytosol. Nuclear CK-2 associated with chromatin is reduced at an even greater rate than that in the total nucleus. Reversal of these events by administration of a single dose of 5alpha-dihydrotestosterone to adult rats castrated 144 hr previously was accompanied by a differential early enhancement of chromatin-associated CK-2 activity, with a concomitant decrease in the CK-2 activity present in the cytosol. Changes in the nuclear CK-2 activity correlated with the immunostainable enzyme protein in the nucleus. We propose that androgens evoke translocation of CK-2 from the cytoplasm to the nucleus (nucleoplasm) where its enhanced association with the chromatin constituents takes place. Conversely, withdrawal of circulating androgens due to castration evokes a dissociation of CK-2 from chromatin and eventual translocation of nucleoplasmic CK-2 to the cytoplasm. Modulations in the association of CK-2 with nuclear chromatin may represent an important mechanism of post-transcriptional regulation of nuclear CK-2 in relation to androgen action in the prostate.