A program for least squares analysis of reassociation and hybridization data.
A program for least squares analysis of reassociation and hybridization data.
复制标题
用于重新关联和杂交数据的最小二乘分析的程序。
作者：
W. Pearson;E. Davidson;R. Britten
描述了一种计算机程序，用于快速计算适合重关联和杂交动力学测量中通常使用的不同函数的数据的最小二乘解。作为 Cot 函数的未反应分数的方程如下： 一阶，exp(-kCot)；二阶，(1+kCot)-1；可变阶数，(1+kCot)-n；保留单链的 DNA 序列的近似分数，(1+kCot)-.44；以及当示踪剂的速率常数 (k) 与驱动器速率常数 (kd) 不同时描述示踪剂配对的函数：（公式：参见文本）。大多数这些功能形式可以使用多个组件。计算解中各个参数的标准偏差。
A computer program is described for the rapid calculation of least squares solutions for data fitted to different functions normally used in reassociation and hybridization kinetic measurements. The equations for the fraction not reacted as a function of Cot follow: First order, exp(-kCot); second order, (1+kCot)-1; variable order, (1+kCot)-n; approximate fraction of DNA sequence remaining single stranded, (1+kCot)-.44; and a function describing the pairing of tracer when the rate constant for the tracer (k) is distinct from the driver rate constant (kd): (formula: see text). Several components may be used for most of these functional forms. The standard deviations of the individual parameters at the solutions are calculated.