PIK3CA amplification and PTEN loss in diffused large B-cell lymphoma.
PIK3CA amplification and PTEN loss in diffused large B-cell lymphoma.
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DOI：
10.18632/oncotarget.19889
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发表时间：
2017-09-12
期刊：
影响因子：
--
通讯作者：
Zhang W
中科院分区：
文献类型：
--
作者：
Cui W;Ma M;Zheng S;Ma Z;Su L;Zhang W
虽然已知在DLBCL中PIK3CA在蛋白水平上扩增，PTEN在蛋白水平上缺失，但在DNA水平上PIK3CA和PTEN基因改变的临床病理意义尚未确定。为了了解PIK3CA和PTEN在DLBCL中遗传状态的临床意义，本研究采用荧光原位杂交（FISH）技术对205例临床样本组织中PIK3CA和PTEN的遗传变化进行了评估。同时，为了了解PIK3CA和PTEN基因改变的临床病理意义，我们采用交叉表分析方法，分析PIK3CA和PTEN基因改变与年龄、性别、体型、体位、国际预后指标、运动状态、b症状、临床分期、外结部位、乳酸脱氢酶浓度、治疗效果、治疗方案及整体预后等临床病理变量的相关性。结果发现，PIK3CA基因在DNA水平上扩增，PTEN基因缺失，扩增率为12.7%(26/205)，缺失率为12.2%（25/205）。此外，没有观察到PIK3CA和PTEN的遗传变化与可用的临床病理变量之间的显著关联。PTEN缺失与PIK3CA扩增之间也没有发现显著相关性。我们的研究结果表明PTEN缺失和PIK3CA在DNA水平上的扩增是DLBCL发病的一个事件。
Although it has been known that PIK3CA was amplified and PTEN was deficient on protein level in DLBCL, the clinicopathological significance of PIK3CA and PTEN genetic change on DNA level hasn’t been established. Here, in our present study, to understand the clinical significance of genetic status of PIK3CA and PTEN in DLBCL, fluorescent in-situ hybridization (FISH) was employed to evaluate the genetic change of PIK3CA and PTEN in clinical sample tissues consist of 205 cases. Incidentally, to understand the clinicopathological significance of genetic change of PIK3CA and PTEN, Cross-table analysis was used to analyze the association between genetic change of PIK3CA and PTEN versus clinicopathological variables available to us, including age, gender, size, location, international prognosis index, performance state, B-symptom, clinical stage, Extra nodal site, concentration of lactate dehydrogenase, therapeutic effects, treatment and overall prognosis. It was found that PIK3CA was amplified and PTEN was deficient on DNA level, the percentage of amplification and loss was 12.7% (26/205) and 12.2% (25/205), respectively. Additionally, no significant association was observed between genetic change of PIK3CA and PTEN versus clinicopathological variables available. Nor was the significant correlation found between loss of PTEN versus PIK3CA amplification. Our results suggest that PTEN deficiency and amplification of PIK3CA on DNA level was an event in the pathogenesis of DLBCL.
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8.8
作者：
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