Cytotoxicity-related alterations of selected cellular functions after in vitro vanadate exposure.
Cytotoxicity-related alterations of selected cellular functions after in vitro vanadate exposure.
复制标题
体外钒酸盐暴露后选定细胞功能的细胞毒性相关改变。
DOI：
10.1016/0006-2952(85)90527-1
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发表时间：
1985
影响因子：
5.8
通讯作者：
R. Sharma
中科院分区：
文献类型：
--
作者：
W. Bracken;R. Sharma
使用牛肾细胞系来监测特定细胞功能的毒性依赖性改变，以检查细胞对钒损伤的反应。使用的钒浓度范围为 20 至 500 μM Na3VO4(V)，引起 15-60% 的细胞毒性。注意到胸苷掺入 DNA 和亮氨酸掺入蛋白质的细胞毒性相关减少。矛盾的是，V 处理的培养物表现出蛋白质和 DNA 含量增加，表明前体转运减少。在存活细胞和无细胞系统中监测 K+ 依赖性磷酸酶 (KP)、酸性磷酸酶 (AP) 和琥珀酸脱氢酶 (SDH)。检测到 KP 和 AP 具有显着的抑制作用； SDH表现出轻微的增强。在无细胞系统中，KP 在 10−7M V 时受到显着抑制，而 AP 和 SDH 仅在 10−4M V 或更高浓度时保持不变或敏感。对存活细胞中还原型谷胱甘肽 (GSH) 的测量显示，毒性依赖性增加高达对照值的 500%。与细胞 V 含量相比，随着细胞存活率的降低，GSH: V 摩尔比从 1.7 降至 0.5。
A bovine kidney cell line was used to monitor select cellular functions for toxicity-dependent alterations in an effort to examine the cellular response to vanadium insult. The vanadium concentrations utilized ranged between 20 and 500 μM Na3VO4(V) and elicited 15–60% cytotoxicity. Cytotoxicity-related decreases in thymidine incorporation into DNA and leucine incorporation into protein were noted. Paradoxically, V-treated cultures exhibited increased protein and DNA content, suggestive of a decrease in precursor transport. K+-dependent phosphatase (KP), acid phosphatase (AP) and succinate dehydrogenase (SDH) were monitored in surviving cells and in a cell-free system. Significant inhibitions were detected for KP and AP; SDH exhibited slight enhancement. In the cell-free system, KP was inhibited significantly at 10−7M V, while AP and SDH were either unchanged or sensitive only at concentrations of 10−4M V or greater. Measurement of reduced glutathione (GSH) in surviving cells revealed toxicity-dependent increases of up to 500% of control values. When compared to the cellular V content, the GSH: V molar ratio decreased from 1.7 to 0.5 as cell survival decreased.
抑制脂质过氧化而不预防离体大鼠肝细胞的细胞损伤。
影响因子：
3.8
作者：
Stacey,NH;Klaassen,CD
通讯作者：
Klaassen,CD