Golden indica and Japonica rice lines amenable to deregulation
Golden indica and Japonica rice lines amenable to deregulation
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DOI：
10.1104/pp.103.023457
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发表时间：
2003-09-01
期刊：
影响因子：
7.4
通讯作者：
Beyer, P
中科院分区：
文献类型：
--
作者：
Hoa, TTC;Al-Babili, S;Beyer, P
作为实现自由获取的重要一步，并因此产生了金稻的影响，发展中国家已经实现了所涉技术的自由经营状况。具体地，为了使本发明超越其在粳稻（Oryza sativa）栽培品种中的初始“概念验证”状态，我们在此报道了两个转化的优良籼稻品种（IR 64和MTL 250）加上一个粳稻品种Taipei 309。籼稻品种主要在缺乏维生素A的地区消费。为了符合监管限制，我们改变了载体骨架，调查的情况下，越界转移，并依赖于根癌农杆菌介导的转化，以获得定义的整合模式。为了避免抗生素选择系统，我们现在完全依赖磷酸甘露糖异构酶作为选择标记。优先考虑单一整合，以尽量减少对后代的潜在表观遗传影响。这些新的品系，现在在T，一代，是非常有价值的，因为他们预计将更容易获得批准的后续研究，如营养和风险评估和育种方法，导致当地适应品种的发展。
As an important step toward free access and, thus, impact of GoldenRice, a freedom-to-operate situation has been achieved for developing countries for the technology involved. Specifically, to carry the invention beyond its initial "proof-of-concept" status in a Japonica rice (Oryza sativa) cultivar, we report here on two transformed elite Indica varieties (IR64 and MTL250) plus one Japonica variety Taipei 309. Indica varieties are predominantly consumed in the areas with vitamin A deficiency. To conform with regulatory constraints, we changed the vector backbone, investigated the absence of beyond-border transfer, and relied on Agrobacterium tumefaciens-mediated transformation to obtain defined integration patterns. To avoid an antibiotic selection system, we now rely exclusively on phosphomannose isomerase as the selectable marker. Single integrations were given a preference to minimize potential epigenetic effects in subsequent generations. These novel lines, now in the T, generation, are highly valuable because they are expected to more readily receive approval for follow-up studies such as nutritional and risk assessments and for breeding approaches leading to locally adapted variety development.