Relationship between CD107a expression and cytotoxic activity
Relationship between CD107a expression and cytotoxic activity
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DOI：
10.1016/j.cellimm.2008.08.007
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Deniz, Gunnur
中科院分区：
文献类型：
--
作者：
Aktas, Esin;Kucuksezer, Umut Can;Deniz, Gunnur
NK细胞在抵抗肿瘤和宿主感染的先天免疫中发挥着重要作用。研究表明，CD107a(LAMP-1)可能是NK和活化的CD8(+)T细胞脱颗粒的标志。本研究探讨了CD56(+)NK、CD8(+)T细胞和淋巴细胞在不同刺激条件下CD107a的表达、细胞因子分泌与细胞毒活性之间的关系。从健康人PBMCs中分离出效应细胞，以K562细胞系为细胞毒作用靶点。IL-2刺激后CID56(+)NK、CD8(+)T细胞和淋巴细胞CD107a表达显著增加。IL-2刺激NK细胞后CD107a表达的增加与细胞毒作用的增强是平行的。我们的结果提示，CD107a的表达可能是检测细胞毒活性的一个敏感指标。(C)2008 Elsevier Inc.保留所有权利。
NK cells play important roles in innate immunity against tumors and infections of the host. Studies show that CD107a (LAMP-1) may be a marker for degranulation of NK and activated CD8(+) T cells. In our study, the relationship between the expression of CD107a, cytokine secretion and cytotoxic activity in CD56(+) NK, CD8(+) T cells and lymphocytes has been determined after various stimuli. Effector cells from PBMCs of healthy subjects were isolated and K562 cell line was used as target of cytotoxicity. IL-2 stimulation resulted in a significant increase of CD107a expression in CID56(+) NK, CD8(+) T cells and lymphocytes. Increased expression of CD107a after IL-2 stimulation of NK cells was parallel to the increase of cytotoxicity. Our results suggest that CD107a expression may be a sensitive marker for the cytotoxic activity determination. (c) 2008 Elsevier Inc. All rights reserved.