Carbon and hydrogen isotopic fractionation during lipid biosynthesis in a higher plant (Cryptomeria japonica)
Carbon and hydrogen isotopic fractionation during lipid biosynthesis in a higher plant (Cryptomeria japonica)
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DOI：
10.1016/j.phytochem.2003.12.003
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发表时间：
2004-02-01
期刊：
影响因子：
3.8
通讯作者：
Poulson, SR
中科院分区：
文献类型：
--
作者：
Chikaraishi, Y;Naraoka, H;Poulson, SR
化合物特定的碳和氢同位素组成的脂质生物分子（正烷烃，正链烷酸，正链烷醇，倍半萜，二萜，植醇，二萜醇和β-谷甾醇），从柳杉叶提取，以了解同位素分馏发生在这个物种的脂质生物合成过程中。相对于散装组织和环境水，所有脂质生物分子在C-13和D两者中分别被耗尽。与产乙酸途径相关的正烷基脂质在C-13中相对于主体组织被消耗2.4- 9.9ppm。相对于环境水体，D的贫化率为91- 152 ppm。与甲羟戊酸途径相关的C-15-和C-30-类异戊二烯脂质（倍半萜、角鲨烯和β-谷甾醇）中的C-13相对于主体组织减少了1.7- 3.1ppm。相对于环境水体，D的贫化率为212- 238 ppm。与非甲羟戊酸途径相关的C-20-类异戊二烯脂质（植醇和二萜类化合物）的13 C相对于主体组织的消耗为4.6- 5.9ppm，D相对于环境水的消耗为238- 303 ppm。植醇中D的含量相对于其他C-20类异戊二烯脂质显著降低，高达65 ppm。产乙酸，甲羟戊酸和非甲羟戊酸途径明确区分使用碳和氢同位素分馏之间的交叉图。(C)2003 Elsevier Ltd.保留所有权利。
Compound-specific carbon and hydrogen isotopic compositions of lipid biomolecules (n-alkanes, n-alkanoic acids, n-alkanols, sesquiterpenes, diterpenes, phytol, diterpenols and beta-sitosterol), extracted from Cryptomeria japonica leaves, were determined in order to understand isotopic fractionations occurring during lipid biosynthesis in this species. All lipid biomolecules were depleted in both C-13 and D relative to bulk tissue and ambient water, respectively. n-Alkyl lipids associated with the acetogenic pathway were depleted in C-13 relative to bulk tissue by 2.4-9.9parts per thousand. and depleted in D relative to ambient water by 91-152parts per thousand. C-15- and C-30- isoprenoid lipids (sesquiterpenes, squalene and beta-sitosterol) associated with the mevalonic-acid pathway are depleted in C-13 relative to bulk tissue by 1.7-3.1parts per thousand. and depleted in D relative to ambient water by 212-238parts per thousand. C-20-isoprenoid lipids (phytol and diterpenoids) associated with the non-mevalonic-acid pathway were depleted in 13C relative to bulk tissue by 4.6-5.9parts per thousand and depleted in D relative to ambient water by 238-303parts per thousand. Phytol was significantly depleted in D by amounts up to 65parts per thousand relative to other C-20 isoprenoid lipids. The acetogenic, mevalonic-acid and non-mevalonic-acid pathways were clearly discriminated using a cross-plot between the carbon and hydrogen isotopic fractionations. (C) 2003 Elsevier Ltd. All rights reserved.