Characterization of P55, a Multidrug Efflux Pump inMycobacterium bovis and Mycobacterium tuberculosis
Characterization of P55, a Multidrug Efflux Pump inMycobacterium bovis and Mycobacterium tuberculosis
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DOI：
10.1128/aac.45.3.800-804.2001
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发表时间：
2001-03
影响因子：
4.9
通讯作者：
Pedro E. A. Silva;F. Bigi;María de la Paz Santangelo;M. Romano;Carlos Martín;A. Cataldi;J. Aínsa
中科院分区：
文献类型：
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作者：
Pedro E. A. Silva;F. Bigi;María de la Paz Santangelo;M. Romano;Carlos Martín;A. Cataldi;J. Aínsa
摘要牛分枝杆菌P55基因位于编码免疫原性脂蛋白P27的基因下游，已被表征。该基因与结核分枝杆菌H37 Rv基因组中Rv 1410 c基因的开放阅读框相同，被注释为可能的药物外排蛋白。与P55基因相似的基因在所有的M.结核复合体和其他分枝杆菌，如麻风分枝杆菌和鸟分枝杆菌。Western blotting显示P55蛋白定位于M.牛。当克隆后转化到耻垢分枝杆菌中时，P55赋予氨基糖苷类和四环素类抗性。在质子载体羰基氰间氯苯腙和泵抑制剂维拉帕米和利血平的存在下，P55赋予的链霉素和四环素的抗性水平降低。M.表达质粒编码的P55的耻垢细胞比对照细胞积累更少的四环素。我们得出的结论是，P55是一种与分支杆菌中氨基糖苷类和四环素外排有关的膜蛋白。
ABSTRACT The Mycobacterium bovis P55 gene, located downstream from the gene that encodes the immunogenic lipoprotein P27, has been characterized. The gene was identical to the open reading frame of the Rv1410c gene in the genome of Mycobacterium tuberculosisH37Rv, annotated as a probable drug efflux protein. Genes similar toP55 were present in all species of the M. tuberculosis complex and other mycobacteria such asMycobacterium leprae and Mycobacterium avium. By Western blotting, P55 was located in the membrane fraction ofM. bovis. When transformed into Mycobacterium smegmatis after cloning, P55 conferred aminoglycoside and tetracycline resistance. The levels of resistance to streptomycin and tetracycline conferred by P55 were decreased in the presence of the protonophore carbonyl cyanide m-chlorophenylhydrazone and the pump inhibitors verapamil and reserpine. M. smegmatiscells expressing the plasmid-encoded P55 accumulated less tetracycline than the control cells. We conclude that P55 is a membrane protein implicated in aminoglycoside and tetracycline efflux in mycobacteria.