Association between Pericytes in Intraplaque Neovessels and Magnetic Resonance Angiography Findings
Association between Pericytes in Intraplaque Neovessels and Magnetic Resonance Angiography Findings
复制标题
DOI：
10.3390/ijms21061980
复制
发表时间：
2020-03
影响因子：
5.6
通讯作者：
Atsushi Ogata;Tomihiro Wakamiya;M. Nishihara;Tatsuya Tanaka;T. Mizokami;J. Masuoka;Nobuaki Momozaki;S. Sakata;H. Irie;T. Abe
中科院分区：
文献类型：
--
作者：
Atsushi Ogata;Tomihiro Wakamiya;M. Nishihara;Tatsuya Tanaka;T. Mizokami;J. Masuoka;Nobuaki Momozaki;S. Sakata;H. Irie;T. Abe
(1)背景：周细胞参与晚期和复杂动脉粥样硬化病变斑块内新生血管的形成。然而，周细胞在人颈动脉斑块中的作用尚不清楚。在飞行时间（TOF）磁共振血管造影（MRA）上显示高强度信号的不稳定颈动脉斑块通常是缺血性中风的原因。本研究的目的是检查斑块内新血管周细胞与MRA结果之间的关系。(2)方法：选取我院行颈动脉内膜切除术的49例颈动脉狭窄患者46例。回顾性分析经组织病理学评估的颈动脉斑块患者。用糖蛋白A染色评估斑块内出血，用CD34（分化簇）染色作为内皮细胞标志物或NG2（神经元胶质抗原2）和CD146染色作为周细胞标志物评估斑块内新生血管。此外，我们还评估了TOF-MRA结果与颈动脉斑块病理之间的关系。(3)结果49例血管狭窄，TOF-MRA高信号28例（TOF-HIS组），等信号21例（TOF-IIS组）。两组cd34阳性新生血管密度相等。然而，在TOF-HIS组中，NG2-和cd146阳性的新生血管密度明显高于TOF-IIS组。(4)结论：颈动脉斑块TOF-MRA上出现高强度信号与斑块内出血及斑块内新生血管周细胞较少有关。这些发现可能有助于开发以周细胞为重点的新治疗策略。
(1) Background: Pericytes are involved in intraplaque neovascularization of advanced and complicated atherosclerotic lesions. However, the role of pericytes in human carotid plaques is unclear. An unstable carotid plaque that shows high-intensity signals on time-of-flight (TOF) magnetic resonance angiography (MRA) is often a cause of ischemic stroke. The aim of the present study is to examine the relationship between the pericytes in intraplaque neovessels and MRA findings. (2) Methods: A total of 46 patients with 49 carotid artery stenoses who underwent carotid endarterectomy at our hospitals were enrolled. The patients with carotid plaques that were histopathologically evaluated were retrospectively analyzed. Intraplaque hemorrhage was evaluated using glycophorin A staining, and intraplaque neovessels were evaluated using CD34 (Cluster of differentiation) stain as an endothelial cell marker or NG2 (Neuron-glial antigen 2) and CD146 stains as pericyte markers. Additionally, the relationships between the TOF-MRA findings and the carotid plaque pathologies were evaluated. (3) Results: Of the 49 stenoses, 28 had high-intensity signals (TOF-HIS group) and 21 had iso-intensity signals (TOF-IIS group) on TOF-MRA. The density of the CD34-positive neovessels was equivalent in both groups. However, the NG2- and CD146-positive neovessels had significantly higher densities in the TOF-HIS group than in the TOF-IIS group. (4) Conclusion: The presence of a high-intensity signal on TOF-MRA in carotid plaques was associated with intraplaque hemorrhage and few pericytes in intraplaque neovessels. These findings may contribute to the development of new therapeutic strategies focusing on pericytes.