Effect of chondrocyte-derived early extracellular matrix on chondrogenesis of placenta-derived mesenchymal stem cells
Effect of chondrocyte-derived early extracellular matrix on chondrogenesis of placenta-derived mesenchymal stem cells
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DOI：
10.1088/1748-6041/10/3/035014
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发表时间：
2015-06-01
影响因子：
4
通讯作者：
Ha, Chul-Won
中科院分区：
文献类型：
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作者：
Park, Yong-Beom;Seo, Sinji;Ha, Chul-Won
细胞周围的细胞外基质（ECM）含有多种蛋白质，提供结构支持并调节细胞功能。先前的研究表明，从组织或培养的细胞中分离的脱细胞ECM可用于在组织工程应用中改善细胞分化。在这项研究中，我们评估了脱细胞软骨细胞来源的ECM（CDECM）对人胎盘来源的间充质干细胞（hPDMSC）在颗粒培养系统中的软骨形成的影响。在软骨形成培养基中与或不与软骨细胞来源的ECM一起孵育1或3周后，将细胞团块的大小和湿质量与未处理的对照（在没有软骨细胞来源的ECM的软骨形成培养基中孵育的hPDMSC）进行比较。此外，进行细胞团块的组织学分析（番红O和II型胶原染色）和软骨形成标志物（聚集蛋白聚糖、II型胶原和SOX 9）的定量逆转录PCR分析。我们的结果表明，与软骨细胞来源的ECM一起孵育的hPDMSC颗粒的大小和质量显著高于未处理的对照组。通过番红0和II型胶原染色证实hPDMSC（具有和不具有软骨细胞来源的ECM）的分化。与未处理的对照组相比，与软骨细胞衍生的ECM一起孵育的hPDMSC团块中的软骨形成标记物表达和糖胺聚糖（GAG）水平显著更高，特别是在与软骨细胞衍生的ECM一起预培养7 d的细胞中。综上所述，这些结果表明，软骨细胞衍生的ECM增强了hPDMSC的软骨形成，并且这种效果通过与软骨细胞衍生的ECM预培养进一步增加。这种hPDMSC软骨形成的预培养方法代表了软骨组织工程的一种有前途的方法。
The extracellular matrix (ECM) surrounding cells contains a variety of proteins that provide structural support and regulate cellular functions. Previous studies have shown that decellularized ECM isolated from tissues or cultured cells can be used to improve cell differentiation in tissue engineering applications. In this study we evaluated the effect of decellularized chondrocytederived ECM (CDECM) on the chondrogenesis of human placenta-derived mesenchymal stem cells (hPDMSCs) in a pellet culture system. After incubation with or without chondrocyte-derived ECM in chondrogenic medium for 1 or 3 weeks, the sizes and wet masses of the cell pellets were compared with untreated controls (hPDMSCs incubated in chondrogenic medium without chondrocytederived ECM). In addition, histologic analysis of the cell pellets (Safranin O and collagen type II staining) and quantitative reverse transcription-PCR analysis of chondrogenic markers (aggrecan, collagen type II, and SOX9) were carried out. Our results showed that the sizes and masses of hPDMSC pellets incubated with chondrocyte-derived ECM were significantly higher than those of untreated controls. Differentiation of hPDMSCs (both with and without chondrocyte-derived ECM) was confirmed by Safranin O and collagen type II staining. Chondrogenic marker expression and glycosaminoglycan (GAG) levels were significantly higher in hPDMSC pellets incubated with chondrocyte-derived ECM compared with untreated controls, especially in cells precultured with chondrocyte-derived ECM for 7 d. Taken together, these results demonstrate that chondrocytederived ECM enhances the chondrogenesis of hPDMSCs, and this effect is further increased by preculture with chondrocyte-derived ECM. This preculture method for hPDMSC chondrogenesis represents a promising approach for cartilage tissue engineering.