Dramatic changes in patterning gene expression during metamorphosis are associated with the formation of a feather-like antenna by the silk moth, Bombyx mori
Dramatic changes in patterning gene expression during metamorphosis are associated with the formation of a feather-like antenna by the silk moth, Bombyx mori
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DOI：
10.1016/j.ydbio.2011.05.672
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发表时间：
2011-09-01
影响因子：
2.7
通讯作者：
Fujiwara, Haruhiko
中科院分区：
文献类型：
--
作者：
Ando, Toshiya;Kojima, Tetsuya;Fujiwara, Haruhiko
许多飞蛾利用性信息素在黑暗中寻找配偶。它们的触角发达，有侧枝，可以有效地接收信息素。然而，这些结构是如何进化的仍然难以捉摸，因为这些触角的发育机制尚未在分子水平上进行研究。为了阐明这种触角的发育机制，我们观察了蚕蛾（Bombyx mod）分枝触角的形态发生、细胞增殖、细胞死亡和触角图案基因表达。区域特异性细胞增殖和几乎普遍存在的细胞凋亡发生在蛹早期，并且似乎共同塑造了侧枝。触角模式基因的表达模式在无分支触角昆虫中基本上保守，直到第 5 龄幼虫龄晚期，但它们中的大多数在变态过程中将其表达急剧改变为预示侧枝的模式。这些发现表明，尽管触角原基在变态之前通过保守机制形成图案，但大多数触角图案基因在变态过程中被重新利用以形成侧枝。我们建议采购一个新的天线调节电路！模式基因可能是鳞翅目具有侧枝的感觉触角进化过程中的一个重要事件。 (C) 2011 Elsevier Inc. 保留所有权利。
Many moths use sex pheromones to find their mates in the dark. Their antennae are well developed with lateral branches to receive the pheromone efficiently. However, how these structures have evolved remains elusive, because the mechanism of development of these antennae has not been studied at a molecular level. To elucidate the developmental mechanism of this type of antenna, we observed morphogenesis, cell proliferation, cell death and antennal patterning gene expression in the branched antenna of the silk moth, Bombyx mod. Region-specific cell proliferation and almost ubiquitous apoptosis occur during early pupal stages and appear to shape the lateral branch cooperatively. Antennal patterning genes are expressed in a pattern largely conserved among insects with branchless antennae until the late 5th larval instar but most of them change their expression dramatically to a pattern prefiguring the lateral branch during metamorphosis. These findings imply that although antennal primordium is patterned by conserved mechanisms before metamorphosis, most of the antennal patterning genes are reused to form the lateral branch during metamorphosis. We propose that the acquisition of a new regulatory circuit of antenna! patterning genes may have been an important event during evolution of the sensory antenna with lateral branches in the Lepidoptera. (C) 2011 Elsevier Inc. All rights reserved.