Cyclic loading increases friction and changes cartilage surface integrity in lubricin-mutant mouse knees
Cyclic loading increases friction and changes cartilage surface integrity in lubricin-mutant mouse knees
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DOI：
10.1002/art.33337
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发表时间：
2012-02-01
影响因子：
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通讯作者：
Crisco, Joseph J.
中科院分区：
文献类型：
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作者：
Drewniak, Elizabeth I.;Jay, Gregory D.;Crisco, Joseph J.
目的研究润滑素基因剂量和循环载荷对小鼠膝关节整体摩擦系数和关节软骨表面完整性的影响。来自具有2(Prg4(+/+))、1(Prg4(+/-))或无(Prg4(-/-))功能润滑素等位基因的小鼠的关节使用定制的钟摆承受26小时的循环载荷。在多个时间点测量摩擦系数。对侧对照关节处于卸载状态。在测试之后，检查关节的组织学损伤证据和细胞存活率。在基线时，Prg4-/-小鼠的摩擦系数显著高于Prg4-/-和Prg4-/-小鼠(P
Objective To investigate the effects of lubricin gene dosage and cyclic loading on whole joint coefficient of friction and articular cartilage surface integrity in mouse knee joints.Methods. Joints from mice with 2 (Prg4(+/+)), 1 (Prg4(+/-)), or no ( Prg4(-/-)) functioning lubricin alleles were subjected to 26 hours of cyclic loading using a custom- built pendulum. Coefficient of friction values were measured at multiple time points. Contralateral control joints were left unloaded. Following testing, joints were examined for histologic evidence of damage and cell viability.Results. At baseline, the coefficient of friction values in Prg4-/- mice were significantly higher than those in Prg4-/- and Prg4-/- mice (P