Biological Effects of Polychromatic Light¶
Biological Effects of Polychromatic Light¶
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DOI：
10.1562/0031-8655(2002)076
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发表时间：
2002-08
期刊：
影响因子：
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通讯作者：
J. Sutherland
中科院分区：
文献类型：
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作者：
J. Sutherland
摘要预测多色光对生物系统的影响是环境光生物学的核心目标。如果一个过程的剂量响应函数是光谱内每个波长入射到系统上的光的线性函数，则通过对每个波长下反应的横截面与该波长下的光谱辐照度在波长和时间上的乘积进行积分，可以获得多色光谱的效果。然而，如果效应的剂量-响应函数不是光子剂量的线性函数，则不能使用该过程。尽管许多光化学反应在生物学相关剂量范围内呈线性，但许多生物学终点并非如此。我描述的程序计算多色辐射对系统表现出某些类的剂量响应函数，包括幂律响应典型的突变诱导和指数剂量响应典型的细胞存活的影响。我还提出了一种方法来预测多色光谱对系统的影响，在该系统中，紫外线成分形成嘧啶二聚体，和较长波长的紫外线和可见光成分去除它们的光复活，从而产生复杂的剂量响应函数，这些耦合的光驱动的反应。
Abstract Predicting the effects of polychromatic light on biological systems is a central goal of environmental photobiology. If the dose–response function for a process is a linear function of the light incident on a system at each wavelength within the spectrum, the effect of a polychromatic spectrum is obtained by integrating the product of the cross section for the reaction at each wavelength and the spectral irradiance at that wavelength over both wavelength and time. This procedure cannot be used, however, if the dose–response functions for an effect are not linear functions of photon dose. Although many photochemical reactions are linear within the biologically relevant range of doses, many biological end points are not. I describe procedures for calculating the effects of polychromatic irradiations on systems that exhibit certain classes of dose–response functions, including power law responses typical of mutation induction and exponential dose–responses typical of cell survival. I also present an approach to predict the effects of polychromatic spectra on systems in which the ultraviolet components form pyrimidine dimers, and the longer-wavelength ultraviolet and visible components remove them by photoreactivation, thus generating complex dose–response functions for these coupled light–driven reactions.