Development of novel detection reagent for simple and sensitive determination of trace amounts of formaldehyde and its application to flow injection spectrophotometric analysis
Development of novel detection reagent for simple and sensitive determination of trace amounts of formaldehyde and its application to flow injection spectrophotometric analysis
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DOI：
10.1016/j.aca.2008.02.028
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发表时间：
2008-04-01
影响因子：
6.2
通讯作者：
Motomizu, Shoji
中科院分区：
文献类型：
--
作者：
Li, Qiong;Sritharathikhum, Piyanete;Motomizu, Shoji
本文提出了一种新型甲醛测定检测试剂，并应用于简单、高灵敏度的流动注射分光光度法测定甲醛。该方法基于甲醛与乙酰乙酸甲酯在氨存在下的反应。在 375 nm 处测量反应产物吸光度的增加。首次使用基于廉价发光二极管 (LED) 的紫外线检测器 (375 nm)。在优化的实验条件下，对0.25～20.0×10(-6)M浓度范围内的水溶液中甲醛进行了测定，并绘制了线性校正图； 5 x 10(-8) M (1.5 mu g L-1) 的检测限 (LOD) 是可能的。 5 X 10(-6) M 甲醛的 12 次重复测量的相对标准偏差为 1.2%。最大采样吞吐量约为 21 个样本/小时。还检查了金属、有机化合物和其他醛等潜在干扰的影响。将甲醛测定的分析性能与使用可见吸收分光光度法的传统乙酰丙酮方法获得的分析性能进行了比较。最后，该方法成功应用于天然水样中甲醛的测定。 (C) 2008 Elsevier B.V. 保留所有权利。
In this paper, a novel detection reagent for formaldehyde determination is proposed, and is applied to a simple and highly sensitive flow injection method for the spectrophotometric determination of formaldehyde. The method is based on the reaction of formaldehyde with methyl acetoacetate in the presence of ammonia. The increase in the absorbance of the reaction product was measured at 375 nm. An inexpensive light emitting diode (LED)based UV detector (375 nm) was, for the first time, used. Under the optimized experimental conditions, formaldehyde in an aqueous solution was determined over the concentration range from 0.25 to 20.0 x 10(-6) M with a liner calibration graph; the limit of detection (LOD) of 5 x 10(-8) M (1.5 mu g L-1) was possible. The relative standard deviation of 12 replicate measurements of 5 X 10(-6) M formaldehyde was 1.2%. Maximum sampling throughput was about 21 samples/h. The effect of potential interferences such as metals, organic compounds and other aldehyde was also examined. The analytical performance for formaldehyde determination was compared with those obtained by the conventional acetylacetone method, which uses visible absorption spectrophotometry. Finally, the proposed method was successfully applied to the determination of formaldehyde in natural water samples. (C) 2008 Elsevier B.V. All rights reserved.