Oxygen deprivation causes suspended animation in the zebrafish embryo
Oxygen deprivation causes suspended animation in the zebrafish embryo
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DOI：
10.1073/pnas.131213198
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发表时间：
2001-06-19
影响因子：
11.1
通讯作者：
Roth, MB
中科院分区：
文献类型：
--
作者：
Padilla, PA;Roth, MB
几乎所有脊椎动物都必须持续接触氧气。我们发现斑马鱼丹尼奥胚胎在缺氧（0% O-2）条件下可存活24 h。斑马鱼进入一种假死状态，所有显微镜下可观察到的运动都停止了，包括细胞分裂、发育进程和运动。在缺氧暴露前有心跳的动物在返回陆地大气（常氧，20.8% O-2）之前没有心跳的证据。我们发现没有细胞在有丝分裂中停滞。这与分期相似的正氧胚胎形成鲜明对比，后者在有丝分裂中始终含有超过15%的细胞。流式细胞术分析显示卵裂球在细胞周期的S期和G(2)期被阻滞。这项研究表明，脊椎动物在缺氧状态下的生存涉及多种过程的减少，如心脏功能和细胞周期进程，从而使能量供应与能量需求相匹配。
Continuous exposure to oxygen is essential for nearly all vertebrates. We found that embryos of the zebrafish Danio rerio can survive for 24 h in the absence of oxygen (anoxia, 0% O-2) In anoxia. zebrafish entered a state of suspended animation where all microscopically observable movement ceased, including cell division, developmental progression, and motility. Animals that had developed a heartbeat before anoxic exposure showed no evidence of a heartbeat until return to terrestrial atmosphere (normoxia, 20.8% O-2) In analyzing cell-cycle changes of: rapidly dividing blastomeres exposed to anoxia. we found that no cells arrested in mitosis. This is in sharp contrast to similarly staged normoxic embryos that consistently contain more than 15% of cells in mitosis. Flow cytometry analysis revealed that blastomeres arrested during the S and G(2) phases of the cell cycle. This work indicates that survival of oxygen deprivation in vertebrates involves the reduction of diverse processes, such as cardiac function and cell-cycle progression, thus allowing energy supply to be matched by energy demands.