A chronic pressure ulcer model in the nude mouse
A chronic pressure ulcer model in the nude mouse
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DOI：
10.1111/j.1524-475x.2009.00502.x
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发表时间：
2009-07-01
影响因子：
2.9
通讯作者：
Redl, Heinz
中科院分区：
文献类型：
--
作者：
Wassermann, Esther;van Griensven, Martijn;Redl, Heinz
压疮的病理生理学尚未完全了解。广泛使用的实验模型仅导致皮肤和皮下组织的压疮。本研究的目的是在裸鼠中开发涉及所有相关组织层的临床相关的深度压疮模型。将Balb/c裸鼠麻醉并将钢盘植入大臀肌下。允许完全恢复10天。然后在未麻醉的小鼠中使用Neoclonal磁体结合圆盘周期性地施加压力（2小时压缩，1小时恢复）。重复周期1、4、6、8或10次。对照组在没有放置磁铁的情况下进行了相同的程序。压疮分级具有周期依赖性，10个周期后最高。组织学评估显示，在四个压缩周期后，皮肤和皮下脂肪出现坏死迹象，在八个和十个压缩周期后，肌肉出现坏死迹象。多形核粒细胞浸润在某些层被认为是依赖于周期数。我们的结论是，这种临床相关的裸鼠模型可用于调查压疮的发展机制，并研究新的治疗方法。
The pathophysiology of pressure ulcers is not yet fully understood. Widely used experimental models lead to pressure ulcers in the skin and the subcutaneous tissue only. The aim of the present study was to develop a clinically relevant deep pressure ulcer model in nude mice involving all relevant tissue layers. Balb/c nude mice were anesthetized and a steel disk was implanted under the great gluteus muscle. Full recovery was allowed for 10 days. Pressure was then periodically applied (2 hours compression, 1 hour recovery) using a Neodymium magnet in conjunction with the disk in the unanesthetized mouse. Cycles were repeated for 1, 4, 6, 8, or 10 times. Controls underwent the same procedure without placement of the magnet. The pressure ulcer grade was found to be cycle dependent,with the highest degree after 10 cycles. Histological evaluations revealed signs of necrosis in the skin and subcutaneous fat after four, and in the muscle after eight and 10 cycles of compression. Polymorphnuclear granulocyte infiltration in certain layers was found to be dependent on the number of cycles. We conclude that this clinically relevant nude mouse model can be used to investigate the mechanism of pressure ulcer development and to study new therapeutic approaches.