Ia-bearing T lymphocytes in man. Their identification and role in the generation of allogeneic helper activity
Ia-bearing T lymphocytes in man. Their identification and role in the generation of allogeneic helper activity
复制标题
人类携带 Ia 的 T 淋巴细胞。
DOI：
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发表时间：
1978
影响因子：
15.3
通讯作者：
A. Gottlieb
中科院分区：
文献类型：
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作者：
S. Fu;N. Chiorazzi;C. Y. Wang;G. Montrazeri;H. Kunkel;H. S. Ko;A. Gottlieb
通过免疫荧光法，用抗分离的双分子Ia结构的兔抗Ia血清，在少量（2-6%）高度纯化的人循环T淋巴细胞上证明了Ia样抗原的存在。Ia+ T淋巴细胞没有表面或细胞内免疫球蛋白。这种Ia+ T细胞群的扩增在某些患者中遇到。Ia抗原也被发现在长期培养的T母细胞与植物血凝素刺激的淋巴细胞产生的条件培养基。此外，在两名白血病患者的外周血中鉴定了Ia抗原染色并形成Eroximab的白血病母细胞。这一证据进一步支持了人类中存在Ia携带T细胞。Ia+ T淋巴细胞群显示含有负责产生同种异体辅助活性的细胞。通过抗Ia抗血清和补体从纯化的T细胞群中消除Ia+淋巴细胞，消除了其在抗原存在下帮助同种异体B细胞制剂在体外产生抗绵羊红细胞的噬斑形成细胞的能力。
The presence of Ia-like antigens was demonstrated on a small population (2-6%) of highly purified human circulating T lymphocytes by immunofluorescence with a rabbit anti-Ia serum raised against the isolated bimolecular Ia structure. The Ia+ T lymphocytes have no surface or intracellular immunoglobulins. The expansion of this Ia+ T- cell population was encountered in certain patients. Ia antigens were also found on T blasts grown in long-term cultures with conditioned medium generated by phytohemagglutinin-stimulated lymphocytes. In addition, leukemia blasts which stained for Ia antigens and formed E rosettes were identified in the peripheral blood of two leukemic patients. This evidence further supports the existence of Ia-bearing T cells in man. The Ia+ T-lymphocyte population was shown to contain cells responsible for the generation of allogeneic helper activity. Elimination of Ia+ lymphocytes from a purified T-cell population by the anti-Ia antiserum and complement abolished its ability to help an allogeneic B-cell preparation to generate plaque-forming cells against sheep erythrocytes in vitro in the presence of the antigen.