Characterization of rpoB mutations in rifampin-resistant Mycobacterium tuberculosis isolates from the Middle East
Characterization of rpoB mutations in rifampin-resistant Mycobacterium tuberculosis isolates from the Middle East
复制标题
DOI：
10.1016/s0732-8893(00)00200-5
复制
发表时间：
2000-12-01
影响因子：
2.9
通讯作者：
Mustafa, AS
中科院分区：
文献类型：
--
作者：
Ahmad, S;Araj, GF;Mustafa, AS
耐利福平的结核分枝杆菌临床分离株rpoB基因突变的性质和频率因地理位置的不同而有很大差异。没有关于从中东国家的患者中分离的临床结核分枝杆菌菌株中特定突变的流行率的信息。本研究对13株耐利福平的结核分枝杆菌和6株敏感的结核分枝杆菌临床分离株进行了检测，以确定rpoB基因的突变。TB试剂盒和DNA测序均扩增出目的DNA。该试剂盒鉴定了所有6株敏感株都是利福平敏感株，扩增出的靶区rpoB基因的DNA序列与野生型序列完全匹配。该试剂盒通过对8株分离株的特定突变检测，鉴定出12株耐药株为利福平耐药株，而其中一株利福平耐药株被鉴定为利福平敏感株。DNA测序证实了这些结果，此外，还导致了对4株利福平耐药株的突变的特异性检测，其中靶区的特定碱基变化无法通过INNO-LIPA Rif确定。结核病试剂盒。大多数耐药株(8/13)涉及rpoB基因第531密码子的碱基改变。在来自希腊和俄罗斯圣彼得堡的大多数利福平耐药株中也报告了rpoB基因531位密码子的突变，但在其他地理位置没有发现。(C)2000 Elsevier Science Inc.保留所有权利。
The nature and frequency of mutations in the rpoB gene of rifampin-resistant clinical Mycobacterium tuberculosis isolates vary considerably according to geographical locations. There is no information on the prevalence of specific mutations in clinical M. tuberculosis strains isolated from patients in Middle-Eastern countries. In this study, 13 rifampin-resistant and 6 susceptible clinical M. tuberculosis isolates were tested for identification and characterization of mutations in the rpoB gene by INNO-LiPA Rif. TB kit and DNA sequencing of the PCR amplified target DNA. The kit identified all six susceptible strains as rifampin-sensitive and the DNA sequence of the amplified rpoB gene in the target region matched perfectly with the wild-type sequence. The kit identified 12 resistant isolates as rifampin-resistant with specific detection of mutations in 8 isolates while one of the rifampin-resistant strain was identified as rifampin-susceptible. DNA sequencing confirmed these results and, in addition, led to the specific detection of mutations in 4 rifampin-resistant isolates in which specific base changes within the target region could not be determined by the INNO-LiPA Rif. TB kit. The majority (8 of 13) of resistant isolates involved base changes at codon 531 of the rpoB gene. Mutations at codon position 531 within the rpoB gene have also been reported in majority of rifampin-resistant strains from Greece and St. Petersburg, Russia but not from other geographical locations. (C) 2000 Elsevier Science Inc. AII rights reserved.