Multicolor Tunable Polymeric Nanoparticle from the Tetraphenylethylene Cage for Temperature Sensing in Living Cells
Multicolor Tunable Polymeric Nanoparticle from the Tetraphenylethylene Cage for Temperature Sensing in Living Cells
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来自四苯乙烯笼的多色可调谐聚合物纳米颗粒用于活细胞中的温度传感
DOI：
10.1021/jacs.9b11544
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发表时间：
2020-01-08
影响因子：
15
通讯作者：
Tang, Ben Zhong
中科院分区：
文献类型：
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作者：
Wang, Zhen;He, Xuewen;Tang, Ben Zhong
开发一种具有良好细胞相容性、易于读出、高分辨率的活体细胞温度传感荧光探针具有重要意义和挑战性。在此，我们成功地合成了发射星状笼型有机温度敏感聚合物，可以组装成纳米粒子在水溶液中。通过级联福斯特共振能量转移（FRET）效应，通过包封不同剂量的客体染料（（4-二甲基氨基-2 '-丁氧基查耳酮（DMBC）和尼罗红（NR）），可以容易地将所获得的纳米颗粒调谐到具有至少0.5 ℃的温度分辨率的全色发射（包括白色光发射）。此外，所述白色发光聚合物杂化纳米颗粒表现出对温度的可逆刺激响应，并且可以通过其具有良好细胞相容性的在白色和橙子发射之间的荧光颜色变化用作活细胞中的温度传感的探针。
It is meaningful but challenging to develop a fluorescent probe for temperature sensing in living cells because it should possess the features of good cytocompatibility, easy read out, and high resolution. Herein, we successfully synthesized emissive star-like cage-based organic temperature-sensitive polymers that can assemble into nanoparticles in aqueous solution. The obtained nanoparticle can be easily tuned to full-color emission (including white light emission) with a temperature resolution of at least 0.5 degrees C by encapsulating different doses of guest dyes ((4-dimethylamino-2'-butoxychalcone (DMBC) and Nile Red (NR)) through a cascade Forster resonance energy transfer (FRET) effect. Moreover, the white light emission polymeric hybrid nanoparticles exhibit reversible stimuli response toward temperature and can be used as probes for temperature sensing in live cells through their fluorescent color variation between white and orange emission with good cytocompatibility.