The genome of the biting midge Culicoides sonorensis and gene expression analyses of vector competence for Bluetongue virus
The genome of the biting midge Culicoides sonorensis and gene expression analyses of vector competence for Bluetongue virus
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咬蠓库蠓基因组及蓝舌病毒载体能力的基因表达分析
DOI：
10.1101/249482
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Morales-Hojas R
中科院分区：
文献类型：
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作者：
Morales-Hojas R
新的基因组技术为载体、病毒和宿主之间相互作用的遗传学提供了新的见解，这导致在控制具有医学重要性的虫媒病毒方面取得了进展。然而，开发非人畜共患虫媒病毒载体的工具和资源仍然被忽视。库蠓属蠓在世界范围内传播一些最重要的野生动物和牲畜虫媒病毒，对经济生产力、健康和福利产生全球性影响。迄今为止，缺乏合适的参考基因组阻碍了这一重要病媒属的基因组分析。本研究对美国蓝舌病病毒（BTV）载体索诺库蚊（culicoides sonorensis）的基因组进行了测序，为这些载体提供了第一个参考基因组。在这项研究中，我们还报道了使用参考基因组对BTV载体能力进行初步转录组学分析。结果该基因组全长189 Mb，由7974个支架组成。利用本研究和之前的研究中产生的转录组学数据，它的注释已经确定了15,612个基因。基因表达分析。本研究发现165个基因在感染BTV的雌性和难愈雌性之间存在差异表达。两个候选基因，谷胱甘肽s -转移酶（gst）和抗病毒基因helicaseski2，先前被认为参与BTV . sonorensis（gst）的载体能力和抑制dsRNA病毒的繁殖（ski2），在本研究中得到证实。结论c。sonorensiss已经能够初步分析载体胜任和难耐个体的基因表达谱。本研究生成的基因组和转录组为今后虫媒病毒传播的研究提供了合适的工具。这些为这些媒介谱系提供了宝贵的资源，这些媒介谱系在2亿多年前从其他主要的双翅目媒介家族中分化出来。该基因组将为其他重要的双翅目病媒家族（包括蚊科和白蛉科）提供有价值的比较数据，并与转录组学数据一起为病媒控制和功能研究提供转基因修饰的潜在靶标。
BackgroundThe new genomic technologies have provided novel insights into the genetics of interactions between vectors, viruses and hosts, which are leading to advances in the control of arboviruses of medical importance. However, the development of tools and resources available for vectors of non-zoonotic arboviruses remains neglected. Biting midges of the genusCulicoidestransmit some of the most important arboviruses of wildlife and livestock worldwide, with a global impact on economic productivity, health and welfare. The absence of a suitable reference genome has hindered genomic analyses to date in this important genus of vectors. In the present study, the genome ofCulicoides sonorensis,a vector of bluetongue virus (BTV) in the USA, has been sequenced to provide the first reference genome for these vectors. In this study, we also report the use of the reference genome to perform initial transcriptomic analyses of vector competence for BTV.ResultsOur analyses reveal that the genome is 189 Mb, assembled in 7974 scaffolds. Its annotation using the transcriptomic data generated in this study and in a previous study has identified 15,612 genes. Gene expression analyses ofC. sonorensisfemales infected with BTV performed in this study revealed 165 genes that were differentially expressed between vector competent and refractory females. Two candidate genes,glutathione S-transferase(gst) and the antiviral helicaseski2, previously recognized as involved in vector competence for BTV inC. sonorensis(gst) and repressing dsRNA virus propagation (ski2), were confirmed in this study.ConclusionsThe reference genome ofC. sonorensishas enabled preliminary analyses of the gene expression profiles of vector competent and refractory individuals. The genome and transcriptomes generated in this study provide suitable tools for future research on arbovirus transmission. These provide a valuable resource for these vector lineage, which diverged from other major Dipteran vector families over 200 million years ago. The genome will be a valuable source of comparative data for other important Dipteran vector families including mosquitoes (Culicidae) and sandflies (Psychodidae), and together with the transcriptomic data can yield potential targets for transgenic modification in vector control and functional studies.