NORMAL MITOCHONDRIAL STRUCTURE AND GENOME MAINTENANCE IN YEAST REQUIRES THE DYNAMIN-LIKE PRODUCT OF THE MGM1 GENE
NORMAL MITOCHONDRIAL STRUCTURE AND GENOME MAINTENANCE IN YEAST REQUIRES THE DYNAMIN-LIKE PRODUCT OF THE MGM1 GENE
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DOI：
10.1007/bf00324678
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发表时间：
1993-07-01
期刊：
影响因子：
2.5
通讯作者：
DESCHENES, RJ
中科院分区：
文献类型：
--
作者：
GUAN, KL;FARH, L;DESCHENES, RJ
本文报道了酵母动力蛋白家族同源基因MGM1的分离与鉴定。MGM1基因位于XV染色体右臂，位于STE4和PTP2之间。序列分析显示，902个残基的开放阅读框能够编码一个分子质量约为101 kDa的蛋白。MGM1缺失导致在富培养基上生长缓慢，在不可发酵碳源上生长失败，以及线粒体DNA缺失。用线粒体基质标记物的抗体观察时，线粒体也出现异常。MGM1编码一种动力蛋白样蛋白，参与酵母中功能性线粒体的繁殖。
The isolation and characterization of MGM1, an yeast gene with homology to members of the dynamin gene family, is described. The MGM1 gene is located on the right arm of chromosome XV between STE4 and PTP2. Sequence analysis revealed a single open reading frame of 902 residues capable of encoding a protein with an approximate molecular mass of 101 kDa. Loss of MGM1 resulted in slow growth on rich medium, failure to grow on non-fermentable carbon sources, and loss of mitochondrial DNA. The mitochondria also appeared abnormal when visualized with an antibody to a mitochondrial-matrix marker. MGM1 encodes a dynamin-like protein involved in the propagation of functional mitochondria in yeast.