Development of discriminatory multiple-locus variable number tandem repeat analysis for Bartonella henselae.
Development of discriminatory multiple-locus variable number tandem repeat analysis for Bartonella henselae.
复制标题
汉赛巴尔通体的鉴别性多位点可变数量串联重复分析的发展。
DOI：
10.1099/mic.0.2006/001164-0
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发表时间：
2007
期刊：
影响因子：
1.5
通讯作者：
N. Haddad
中科院分区：
文献类型：
--
作者：
M. Monteil;B. Durand;R. Bouchouicha;E. Petit;B. Chomel;M. Arvand;H. Boulouis;N. Haddad
汉赛巴尔通体是一种人畜共患细菌，可感染猫和人类。为流行病学目的开发分型技术已作了几次尝试;然而，开发的大多数技术似乎没有足够的区分性或不容易使用。为了建立B的多位点可变数目串联重复序列（VNTR）分析（MLVA）。henselae中，从参考菌株Houston 1（H1）的基因组序列中选择30个VNTR候选物。VNTR候选物最初通过PCR在6个B上测试。henselae从不同的地理区域分离。从显示两个或更多个等位基因的那些中选择五个VNTR。这五个B。对42只猫B进行了Henselae VNTR（BHV）试验。来自法国（23株分离株）、丹麦（17株分离株）、菲律宾（1株分离株）和美国（F1株）的汉赛氏菌分离株和菌株，来自德国的1株人分离株和H1参考菌株。这些BHV具有足够的区分性，在44个B分离株和菌株中获得31个不同的谱（对应于两个不同的组）。测试过的Henselae使用这些BHV和两个变异等位基因获得了35个图谱。5个标记的组合导致多样性指数为0.98。在猫F1菌株上证明了5种BHV的稳定性，在2、21和41次传代后未观察到稳定性变化。这被认为是对B进行的第一项研究。Henselae分型使用MLVA，它证明了高质量的区分B之间的这种技术。汉赛氏菌分离物。
Bartonella henselae is a zoonotic bacterium that infects cats and humans. Several attempts have been made to develop typing techniques for epidemiological purposes; however, most of the techniques developed do not appear to be sufficiently discriminatory or easy to use. In order to develop multilocus variable number tandem repeat (VNTR) analysis (MLVA) for B. henselae, 30 VNTR candidates were selected from the genome sequence of the reference strain Houston 1 (H1). The VNTR candidates were initially tested by PCR on six B. henselae isolates from different geographical areas. Five VNTRs were selected from those that showed two or more alleles. These five B. henselae VNTRs (BHVs) were tested on 42 feline B. henselae isolates and strains from France (23 isolates), Denmark (17 isolates), the Philippines (one isolate) and the USA (F1 strain), on one human isolate from Germany, and on the H1 reference strain. These BHVs were sufficiently discriminatory to obtain 31 different profiles (corresponding to two different groups) among the 44 isolates and strains of B. henselae tested. Thirty-five profiles were obtained using these BHVs and two variant alleles. The combination of the five markers led to a diversity index of 0.98. The stability of the five BHVs was demonstrated on the feline F1 strain, with no change in stability observed after 2, 21 and 41 passages. This is believed to be the first study conducted on B. henselae typing using MLVA, and it demonstrates the high quality of this technique for discriminating between B. henselae isolates.
影响因子：
158.5
作者：
Koehler, JE;Sanchez, MA;Tappero, JW
通讯作者：
Tappero, JW