Surgical bone marrow aspiration in Aotus lemurinus griseimembra.
Surgical bone marrow aspiration in Aotus lemurinus griseimembra.
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Aotus lemurinus griseimembra 的手术骨髓抽吸。
DOI：
10.1111/j.1600-0684.2006.00151.x
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Herrera,Socrates
中科院分区：
文献类型：
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作者：
Llanos,Cesar;Quintero,Gustavo;Castellanos,Alejandro;Arevalo-Herrera,Myriam;Herrera,Socrates
灰狐猴（Aotus lemurinus griseimembra）对人类疟疾寄生虫感染高度敏感，并复制其一些临床表现，包括贫血。我们开发了一种新的手术技术，通过股骨手术抽吸获得Aotus的骨髓样本。首先，我们确定股骨比其他骨骼具有优势，主要是由于骨折脆弱性较低。我们在甲醛保存的动物中使用20 G IV导管测试了手术技术，然后在27只活体动物中进行了手术。 这种技术提供了简单、快速的手术途径，可以获得足够量的骨髓，而且几乎对所有动物都是安全的：只有一只动物死亡;另一只动物出现了下肢神经损伤。在所有动物中获得足够的细胞样本，并允许进行细胞学研究。这一程序为Aotus的骨髓研究提供了一个有用的工具，并有助于克服目前人类骨髓研究的局限性，这些研究受到伦理和技术问题的限制。
Aotus lemurinus griseimembraare highly susceptible to infection by human malaria parasites and reproduce some of its clinical manifestations, including anemia. We developed a new surgical technique to obtain bone marrow samples fromAotusby surgical aspiration of the femur. First, we determined that the femur offered advantages over other bones, primarily due to lower fracture vulnerability. We tested a surgical technique using 20 G IV catheters in formaldehyde‐preserved animals, then conducted the procedure on 27 live animals. This technique provided easy, quick surgical access to adequate volumes of bone marrow and was safe for almost all animals: only one died; another developed nervous impairment of the lower limb. Adequate cell samples were obtained in all animals and allowed cytological studies. This procedure offers a useful tool for bone marrow research inAotusand helps overcome current limitations of such research in human where these studies are limited by ethical and technical issues.