Protooncogene expression identifies a transient columnar organization of the forebrain within the late embryonic ventricular zone.
Protooncogene expression identifies a transient columnar organization of the forebrain within the late embryonic ventricular zone.
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原癌基因的表达鉴定了胚胎晚期脑室区内前脑的短暂柱状组织。
DOI：
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发表时间：
1989
影响因子：
11.1
通讯作者：
D. van der Kooy
中科院分区：
文献类型：
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作者：
J. G. Johnston;D. van der Kooy
用针对致癌多肽的单抗进行的免疫细胞化学研究显示，致癌多肽在胚胎第18天大鼠前脑的脑室带内分布不均。Sis、src、ras和myc编码的多肽集中在相同的分离的5-25个放射状胶质细胞簇中(也通过波形蛋白染色鉴定)，为脑室区域提供了短暂的柱状分隔。与非柱状区相比，在原癌基因染色的放射状胶质细胞柱内可观察到更多的[~3H]胸腺嘧啶核苷标记的室区细胞。放射状胶质细胞的柱状异质性揭示了胚胎脑室带的嵌合性及其细胞的分化增殖。
Immunocytochemical studies using monoclonal antibodies directed against oncogenic peptides revealed a heterogeneous distribution of the peptides within the ventricular zone of the embryonic day 18 rat forebrain. The sis-, src-, ras-, and myc-encoded peptides were concentrated in the same isolated clusters of 5-25 radial glial cells (also identified by vimentin staining), providing a transient columnar compartmentalization to the ventricular zone. An increased number of [3H]thymidine-labeled ventricular zone cells were observed within the protooncogene stained radial glial cell columns as compared to noncolumn areas. The columnar heterogeneity of radial glial cells reveals the mosaicism of the embryonic ventricular zone and the differential proliferation of its cells.