Transcriptome and gene expression analysis during flower blooming in Rosa chinensis 'Pallida'
Transcriptome and gene expression analysis during flower blooming in Rosa chinensis 'Pallida'
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月季花开花过程中的转录组和基因表达分析
DOI：
10.1016/j.gene.2014.02.008
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发表时间：
2014
期刊：
影响因子：
3.5
通讯作者：
Tang Kaixue
中科院分区：
文献类型：
--
作者：
Yan Huijun;Zhang Hao;Chen Min;Jian Hongying;Baudino Sylvie;Caissard Jean-Claude;Bendahmane Mohammed;Li Shubin;Zhang Ting;Zhou Ningning;Qiu Xianqin;Wang Qigang;Tang Kaixue
月季Rosa chinensis 'Pallida'（RosaL.）是原产于中国的最重要的古月季品种之一。它为现代玫瑰提供了“茶香”的特征。然而，有关蔷薇属植物气味合成和代谢的基因调控网络的信息很少。在本研究中，R.利用Illumina测序技术，研究了从花蕾到衰老花的不同发育阶段的“Pallida”花瓣的DNA序列。从头组装产生89，614个簇，平均长度为428 bp。基于与已知蛋白质的序列相似性搜索，总聚类的62.9%被注释。在这些注释的转录本中，分别有25，705和37，159个序列被分配到基因本体和直链组簇。该数据集提供了与已知气味代谢途径相关的转录本puerectin的信息。使用来自花芽、开放花和衰老花阶段的RNA样品获得数字基因表达（DGE）。比较DGE和定量真实的时间PCR允许识别的五个转录本编码蛋白质pupillin与气味的生物合成在玫瑰。该研究为进一步发现玫瑰花中与气味相关的基因奠定了基础。
Rosa chinensis ‘Pallida’ (RosaL.) is one of the most important ancient rose cultivars originating from China. It contributed the ‘tea scent’ trait to modern roses. However, little information is available on the gene regulatory networks involved in scent biosynthesis and metabolism inRosa. In this study, the transcriptome ofR. chinensis‘Pallida’ petals at different developmental stages, from flower buds to senescent flowers, was investigated using Illumina sequencing technology. De novo assembly generated 89,614 clusters with an average length of 428 bp. Based on sequence similarity search with known proteins, 62.9% of total clusters were annotated. Out of these annotated transcripts, 25,705 and 37,159 sequences were assigned to gene ontology and clusters of orthologous groups, respectively. The dataset provides information on transcripts putatively associated with known scent metabolic pathways. Digital gene expression (DGE) was obtained using RNA samples from flower bud, open flower and senescent flower stages. Comparative DGE and quantitative real time PCR permitted the identification of five transcripts encoding proteins putatively associated with scent biosynthesis in roses. The study provides a foundation for scent-related gene discovery in roses.