THE ROLE OF THE EAE GENE OF ENTEROHEMORRHAGIC ESCHERICHIA-COLI IN INTIMATE ATTACHMENT IN-VITRO AND IN A PORCINE MODEL
THE ROLE OF THE EAE GENE OF ENTEROHEMORRHAGIC ESCHERICHIA-COLI IN INTIMATE ATTACHMENT IN-VITRO AND IN A PORCINE MODEL
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DOI：
10.1172/jci116718
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发表时间：
1993-09-01
影响因子：
15.9
通讯作者：
KAPER, JB
中科院分区：
文献类型：
--
作者：
DONNENBERG, MS;TZIPORI, S;KAPER, JB
肠致病性大肠杆菌（EPEC）的eaeA基因是其在体外与上皮细胞紧密粘附所必需的。肠出血性大肠大肠杆菌（EHEC）菌株也具有eae基因，并且能够在体外和动物模型中紧密附着和微绒毛消失。为了评估EHEC eae基因在亲密连接中的作用，我们通过线性电穿孔重组等位基因在野生型EHEC O 157：H7菌株86-24中构建了eae缺失/插入突变。所获得的突变体是缺乏在HEp-2细胞中诱导f-肌动蛋白积累，并且在新生仔猪感染模型中不能紧密附着于结肠上皮细胞。当EHEC eae基因或EPEC的eaeA基因被引入质粒上的突变体中时，体内紧密附着恢复。这些结果表明eae基因是EHEC在体内紧密附着所必需的。此外，EPEC基因座实现的互补表明EHEC的eae基因和EPEC的eaeA基因在功能上是同源的。
The eaeA gene of enteropathogenic Escherichia coli (EPEC) is necessary for intimate attachment to epithelial cells in vitro. Enterohemorrhagic E. coli (EHEC) strains also possess an eae gene and are capable of intimate attachment and microvillus effacement in vitro and in animal models. To assess the role of the EHEC eae gene in intimate attachment, we constructed an eae deletion/insertion mutation in wild-type EHEC O157:H7 strain 86-24 by using linear electroporation of a recombinant allele. The mutant obtained was deficient in inducing f-actin accumulation in HEp-2 cells and was incapable of attaching intimately to colonic epithelial cells in a newborn piglet model of infection. Intimate attachment in vivo was restored when the EHEC eae gene or the eaeA gene of EPEC was introduced into the mutant on a plasmid. These results indicate that the eae gene is necessary for intimate attachment of EHEC in vivo. In addition, the complementation achieved by the EPEC locus indicates that the eae gene of EHEC and the eaeA gene of EPEC are functionally homologous.