Reorganization of lamina-associated domains in early mouse embryos is regulated by RNA polymerase II activity.
Reorganization of lamina-associated domains in early mouse embryos is regulated by RNA polymerase II activity.
复制标题
早期小鼠胚胎中核纤层相关结构域的重组受 RNA 聚合酶 II 活性的调节。
DOI：
10.1101/gad.350799.123
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发表时间：
2023
影响因子：
10.5
通讯作者：
Torres-Padilla,Maria-Elena
中科院分区：
文献类型：
--
作者：
Pal,Mrinmoy;Altamirano-Pacheco,Luis;Schauer,Tamas;Torres-Padilla,Maria-Elena
哺乳动物的受精伴随着染色质重塑和核组织的重大变化。胚胎发生的最早事件，包括母体到合子转变过程中的合子基因组激活（ZGA），如何影响这种重塑尚不清楚。在这里，我们研究了核结构的建立，重点是在这种转变过程中层相关结构域（LADs）的重塑。我们报道了LADs在两细胞胚胎中逐渐重组，阻断ZGA会导致细胞核组织的重大变化，包括LADs的染色质和基因组特征的改变以及H3K4me3向核层的重新分布。我们的数据表明，LADs的重排是母体到合子转变的一个组成部分，并且转录有助于形成哺乳动物发育初期的核组织。
Fertilization in mammals is accompanied by an intense period of chromatin remodeling and major changes in nuclear organization. How the earliest events in embryogenesis, including zygotic genome activation (ZGA) during maternal-to-zygotic transition, influence such remodeling remains unknown. Here, we have investigated the establishment of nuclear architecture, focusing on the remodeling of lamina-associated domains (LADs) during this transition. We report that LADs reorganize gradually in two-cell embryos and that blocking ZGA leads to major changes in nuclear organization, including altered chromatin and genomic features of LADs and redistribution of H3K4me3 toward the nuclear lamina. Our data indicate that the rearrangement of LADs is an integral component of the maternal-to-zygotic transition and that transcription contributes to shaping nuclear organization at the beginning of mammalian development.