Semen Parameters of Fertile Guinea Pigs (Cavia porcellus) Collected by Transrectal Electroejaculation
Semen Parameters of Fertile Guinea Pigs (Cavia porcellus) Collected by Transrectal Electroejaculation
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DOI：
10.3390/ani10050767
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发表时间：
2020-05-01
期刊：
影响因子：
3
通讯作者：
Echevarria, Luisa
中科院分区：
文献类型：
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作者：
Benavides, Fernando;Sutovsky, Peter;Echevarria, Luisa
在一些南美国家，豚鼠是一种重要的家畜品种。自2000年以来，秘鲁的豚鼠出口仍在增加。自1986年以来，秘鲁已经对该物种进行了基因改良。然而，生殖生物技术方面的进展很少。因此，缺乏关于该物种无害、高效的精液收集技术的信息。因此，由于缺乏有效的精液参数标准，选择有生育能力的男性需要很长时间。因此，有必要通过一种客观的电射精技术为豚鼠设定精液参考值。这项研究描述了有生育能力的男性的精液参数，并验证了该物种的一种新的精液收集技术。这些值将作为发现不育雄性和选择最佳雄性进行育种的参考，提高农场的繁殖性能。摘要豚鼠作为一种家畜物种，在秘鲁及其周边国家仍在发展和生长，从2000年以来其出口不断增加就可以看出这一点。然而，由于缺乏精液参数标准和安全的精液收集技术，选择已被证明具有生育能力的男性是乏味的。因此，男性的选择需要进行怀孕检测或活产。本研究的目的是描述有生育能力的雄性豚鼠的定性和定量精液参数，设定参考值，并验证一种适用于该物种的新的电射精技术。每周从16名有生育能力的男性身上收集精液。每个男性进行了四次经直肠电射精，采集成功率为95%，产生了39个可存活的精液样本。精液特征如下：PH7.0+/-0.13；射精量0.67+/-0.55毫升；精子活动率90.81+/-6.64%；精子密度36.7+/-28.41×106精子/毫升；精子数20.09+/-17.56×106精子/射精量；形态异常率18.26+/-8.52%；泛素化精子百分率5.57+/-6.28%。这些值将为快速发现最佳繁殖和不育雄性提供参考。所描述的技术可由商业生产者重现。
Simple Summary The guinea pig is an important livestock species in some South American countries. Peruvian guinea pig exports are still increasing since 2000. Peru has made genetic improvements on the species since 1986. However, there are few advances in reproductive biotechnology. Thus, there is a lack of information about harmless, efficient semen collection techniques in the species. Consequently, the selection of fertile males takes a long time due to the absence of validated seminal parameter standards. For that reason, it is necessary to set semen reference values through an objective electroejaculation technique for guinea pigs. This study describes semen parameters of fertile males and validates a novel semen collection technique for the species. These values will serve as a reference to detect infertile males and to select the best males for breeding purposes, improving the reproductive performance on farms.Abstract The guinea pig, as a livestock species, is still developing and growing throughout Peru and neighboring countries, as reflected by its increasing export since 2000. However, the selection of proven fertile males is tedious due to the absence of seminal parameter standards and the lack of safe semen collection techniques. Thus, pregnancy detection or live births are required for males' selection. The purpose of this study was to describe the qualitative and quantitative semen parameters of fertile guinea pig males, to set reference values, and to validate a novel electroejaculation technique for the species. Semen was collected at weekly intervals from sixteen fertile males. Four transrectal electroejaculations were performed per male with 95% successful collections, yielding 39 viable semen samples. Seminal characteristics were as follows: pH 7.0 +/- 0.13; ejaculate volume 0.67 +/- 0.55 mL; sperm motility 90.81 +/- 6.64%; sperm concentration 36.7 +/- 28.41 x 106 sperm/mL; sperm count 20.09 +/- 17.56 x 106 sperm/ejaculate; percentage of abnormal morphology 18.26 +/- 8.52%; and percentage ubiquitinated spermatozoa 5.57 +/- 6.28%. These values will serve as a reference to detect best breeding and infertile males rapidly. The described techniques are reproducible by commercial producers.