Dynamics of nuclear architecture during early embryonic development and lessons from liveimaging.
Dynamics of nuclear architecture during early embryonic development and lessons from liveimaging.
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DOI：
10.1016/j.devcel.2023.02.018
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发表时间：
2023-03
影响因子：
11.8
通讯作者：
Federico Pecori;M. Torres-Padilla
中科院分区：
文献类型：
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作者：
Federico Pecori;M. Torres-Padilla
核组织已经成为基因组功能的潜在关键调节器。在发育过程中，转录程序的部署必须与细胞分裂紧密协调，并且通常伴随着表达基因库的重大变化。这些转录和发育事件被染色质景观的变化所掩盖。许多研究揭示了它们背后的核组织动力学。此外，基于实时成像的方法学的进步使得能够以高空间和时间分辨率研究核组织。在这篇评论中，我们总结了目前的知识，在早期胚胎发生的各种模型系统的核结构的变化。此外，为了突出整合固定细胞和活的方法的重要性，我们讨论了如何不同的活成像技术可以应用于检查核过程及其对我们理解早期发育中的转录和染色质动力学的贡献。最后，我们为这一领域的未决问题提供了未来的途径。
Nuclear organization has emerged as a potential key regulator of genome function. During development, the deployment of transcriptional programs must be tightly coordinated with cell division and is often accompanied by major changes in the repertoire of expressed genes. These transcriptional and developmental events are paralleled by changes in the chromatin landscape. Numerous studies have revealed the dynamics of nuclear organization underlying them. In addition, advances in live-imaging-based methodologies enable the study of nuclear organization with high spatial and temporal resolution. In this Review, we summarize the current knowledge of the changes in nuclear architecture in the early embryogenesis of various model systems. Furthermore, to highlight the importance of integrating fixed-cell and live approaches, we discuss how different live-imaging techniques can be applied to examine nuclear processes and their contribution to our understanding of transcription and chromatin dynamics in early development. Finally, we provide future avenues for outstanding questions in this field.