Regional differences of the pancreatic islet.
Regional differences of the pancreatic islet.
复制标题
胰岛的区域差异。
作者：
S. Bencosme;E. Liepa
Simard(1945)发现，在一只按Ivy和Farrell(1926)方法进行手术的狗的皮下胰腺移植中，A细胞几乎完全消失。我们对A细胞与胰高血糖素的关系的兴趣促使我们研究了西马德]S的工作提供的可能性。在目前的研究中，我们证实了Simard]S的观察结果，即移植物中没有A细胞，并得出结论，胰岛细胞组成的地区差异是移植物中没有A细胞的原因。材料与方法实验动物为8只体重在20~50磅之间的犬和2只成年猫。动物被Nembutal杀死，胰腺被完全切除。除非另有说明，所有的组织都固定在Zenker-Formol中，石蜡包埋，在2.5m处切开，一些切片按照前面描述的程序进行马森[S三色和小森]S铬铝苏木精染色(Bencosme，1952)
Simard (1945) found an almost complete disappearance of the A cell in a subcutaneous pancreatic transplant of one dog which had been operated upon following the method of Ivy and Farrell (1926). Our interest in the A cell in relation to Glucagon led us to investigate the possibilities offered by Simard]s work. In the present study we have confirmed Simard]s observations that the transplanted segment was devoid of A cells and have concluded that regional differences in islet cell composition were responsible for the absence of A cells in the transplants. MATERIAL AND METHODS Eight dogs weighing between 20 and 50 pounds and two mature cats were used. Animals were killed with nembutal and their pancreas completely removed. All tissues unless otherwise specified, were fixed in Zenker-Formol, embedded in paraffin, and cut at 2.5 m Some sections were stained with Masson]s trichrome and Gomori]s Chrome Alum hematoxylin following a procedure previously described (Bencosme, 1952)