Anti-influenza activity of berberine improves prognosis by reducing viral replication in mice
Anti-influenza activity of berberine improves prognosis by reducing viral replication in mice
复制标题
小檗碱的抗流感活性通过减少小鼠病毒复制来改善预后
DOI：
10.1002/ptr.6196
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发表时间：
2018-12-01
影响因子：
7.2
通讯作者：
Jiang, Zhen-You
中科院分区：
文献类型：
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作者：
Yan, Yu-Qi;Fu, Ying-Jie;Jiang, Zhen-You
小檗碱是一种从小檗属植物中分离出来的天然异喹啉类生物碱，具有广泛的生物特性，如抗炎、抗菌、抗真菌和驱虫作用。我们在体内和体外评价了小檗碱对甲型流感/FM1/1/47 （H1N1）的抗病毒作用。结果表明，小檗碱能明显抑制A549细胞和小鼠肺中的病毒复制。同时，与给药小鼠相比，小檗碱能减轻小鼠肺部炎症，减少病毒感染引起的坏死、炎症细胞浸润和肺水肿。小檗碱在mRNA和蛋白水平上抑制病毒感染诱导的TLR7信号通路，如TLR7、MyD88和NF-kappa B （p65）的上调。此外，小檗碱显著抑制病毒感染诱导的Th1/Th2和Th17/Treg比率的升高以及炎症细胞因子的产生。我们的数据通过其抗病毒活性为小檗碱作为病毒感染治疗剂的潜力提供了新的见解。
Berberine, a natural isoquinoline alkaloid isolated from the berberis species, has a wide array of biological properties such as anti-inflammatory, antibacterial, antifungal, and antihelminthic effects. We evaluated the antiviral effect of berberine against influenza A/FM1/1/47 (H1N1) in vivo and in vitro. The results showed that berberine strongly suppressed viral replication in A549 cells and in mouse lungs. Meanwhile, berberine relieved pulmonary inflammation and reduced necrosis, inflammatory cell infiltration, and pulmonary edema induced by viral infection in mice when compared with vehicle-treated mice. Berberine suppressed the viral infection-induced up-regulation of TLR7 signaling pathway, such as TLR7, MyD88, and NF-kappa B (p65), at both the mRNA and protein levels. Furthermore, berberine significantly inhibited the viral infection-induced increase in Th1/Th2 and Th17/Treg ratios as well as the production of inflammatory cytokines. Our data provide new insight into the potential of berberine as a therapeutic agent for viral infection via its antiviral activity.