THE TERMINUS OF SV40 DNA-REPLICATION AND TRANSCRIPTION CONTAINS A SHARP SEQUENCE-DIRECTED CURVE
THE TERMINUS OF SV40 DNA-REPLICATION AND TRANSCRIPTION CONTAINS A SHARP SEQUENCE-DIRECTED CURVE
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DOI：
10.1016/0092-8674(88)90466-7
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发表时间：
1988-02-26
期刊：
影响因子：
64.5
通讯作者：
GRIFFITH, JD
中科院分区：
文献类型：
--
作者：
HSIEH, CH;GRIFFITH, JD
我们已经检查了核小体定位在两个DNA片段包含尖锐的序列定向曲率。在pBR325的两个位点上克隆了一段223 bp的束状Crithidia fasciculata DNa。发现这些位点选择性地重建核小体的效率比相邻的直DNA高5- 7倍。SV40 DNA的复制末端和转录末端都包含在.apprx区域内。200 bp位于1216 bp片段的中心。该片段在电子显微镜下的可视化显示了。apprx的尖锐曲线。200.学位。在终端区域。用该片段重组组蛋白显示，在末端区域组装核小体的可能性比相邻DNA高2至5倍。
We have examined nucleosome positioning on two DNA segments containing sharp sequence-directed curvatures. A 223 bp DNa from Crithidia fasciculata was cloned into two sites in pBR325 separated by 28%. These sites were found to selectively reconstitute nucleosomes 5- to 7-fold more effectivelythan the adjoining straight DNA. The terminus of replication and termini of transcription of SV40 DNA are contained within a region of .apprx. 200 bp centrally located in a 1216 bp fragment. Visualization of this fragment by electron microscopy revealed a sharp curve of .apprx. 200.degree. in the terminal region. Reconstitution of histone protein with this fragment revealed a 2- to 5-fold higher probability of assembling nucleosomes in the terminal region over the adjacent DNA.