KAI1 expression is up-regulated in early pancreatic cancer and decreased in the presence of metastases.
KAI1 expression is up-regulated in early pancreatic cancer and decreased in the presence of metastases.
复制标题
DOI：
--
复制
发表时间：
1996-11
期刊：
影响因子：
11.2
通讯作者：
Xiaozhong Guo;H. Friess;H. Graber;M. Kashiwagi;A. Zimmermann;M. Korc;M. Büchler
中科院分区：
文献类型：
--
作者：
Xiaozhong Guo;H. Friess;H. Graber;M. Kashiwagi;A. Zimmermann;M. Korc;M. Büchler
KAI 1是位于染色体11p11.2-13上的前列腺癌转移抑制基因。应用北方杂交和原位杂交技术，对14例正常胰腺和27例原发性胰腺癌组织中KAI 1 mRNA的表达进行了研究，并将其与患者的临床和病理参数进行了相关性分析。北方印迹分析显示，27例胰腺癌样本中有24例（89%）KAI 1 mRNA表达的稳态水平增加。原位杂交显示82%的胰腺癌组织中KAI 1 mRNA表达增强。癌细胞周围的间质和癌细胞附近的正常胰腺组织显示出非常低的KAI 1 mRNA表达水平。通过北方印迹分析获得的mRNA水平与患者的临床参数的相关性显示，与存在淋巴结或远处转移的晚期肿瘤阶段（III、IV）相比，KAI 1 mRNA水平在早期肿瘤阶段（I、II）中显著更高（P < 0.01）。低分化组KAI 1 mRNA表达水平明显高于中、高分化组（P < 0.05）。我们的研究结果表明KAI 1 mRNA的表达在晚期肿瘤患者中降低。这表明KAI 1表达的减少可能使胰腺癌细胞在淋巴结和远处器官中扩散。
KAI1 is a metastasis suppressor gene for prostate cancer that is located on chromosome 11p11.2-13. Using Northern blot analysis and in situ hybridization, we studied expression of KAI1 mRNA in specimens from 14 normal pancreases and 27 primary pancreatic cancers, and then correlated the findings with the clinical and histopathological parameters of the patients. Northern blot analysis showed increased steady-state levels of KAI1 mRNA expression in 24 of 27 (89%) pancreatic cancer samples. In situ hybridization showed enhanced KAI1 mRNA levels in the pancreatic cancer cells in 82% cancer tissues. The stroma surrounding the cancer mass and normal pancreatic tissue adjacent to the cancer cells exhibited very low levels of KAI1 mRNA expression. Correlation of the mRNA levels obtained by Northern blot analysis with clinical parameters of the patients revealed that KAI1 mRNA levels were significantly higher (P < 0.01) in earlier tumor stages (I, II), compared with advanced tumor stages (III, IV) in which lymph node or distant metastases were present. Furthermore, poorly differentiated tumors had significantly higher KAI1 mRNA levels than those that were moderately or well differentiated (P < 0.05). No association between KAI1 expression and survival was found. Our results indicate that KAI1 mRNA expression is reduced in patients with advanced tumor stages. This suggests that reduction of KAI1 expression might enable pancreatic cancer cells to spread in lymph nodes and to distant organs.