Differential regulation of multiple c-erbA expression by thyrotropin, insulin and insulin-like growth factor I in rat thyroid FRTL-5 cells.
Differential regulation of multiple c-erbA expression by thyrotropin, insulin and insulin-like growth factor I in rat thyroid FRTL-5 cells.
复制标题
促甲状腺素、胰岛素和胰岛素样生长因子 I 对大鼠甲状腺 FRTL-5 细胞中多种 c-erbA 表达的差异调节。
DOI：
10.1016/0303-7207(92)90033-3
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发表时间：
1992
影响因子：
4.1
通讯作者：
Chae,CB
中科院分区：
文献类型：
--
作者：
Kamikubo,K;Nayfeh,SN;Chae,CB
在大鼠甲状腺 FRTL-5 细胞中研究了多种 c-erbAgene 表达的调节。通过Northern印迹分析鉴定了两种oferbAα（α1和α2）mRNA和一种oferbAβ（β1）mRNA。从完全培养基中去除促甲状腺素(TSH)、胰岛素和血清导致α1和α2erbAmRNA水平增加，但不改变erbAβ1 mRNA水平。重新添加 TSH,N6,2'-O-二丁酰 cAMP 或毛喉素会导致 3-12 小时内 α1 和 α2 mRNA 水平短暂降低 (75-90%)。 24小时后α1和α2 mRNA水平恢复。 TSH 作用呈剂量依赖性，在 10−9M 左右显示半最大效应。重新添加 TSH 对 β1 mRNA 水平没有任何影响。 TSH 的作用并不依赖于持续的蛋白质合成，而是需要持续的转录。甲状腺激素生物合成抑制剂、丙基硫氧嘧啶和甲基硫基咪唑对 TSH 作用没有显示出任何影响。重新添加胰岛素或胰岛素样生长因子 I (IGF-I) 会导致 α1 和 α2 mRNA 水平呈剂量依赖性降低，但对 β1 mRNA 水平没有任何影响。它们的作用比 TSH 慢且持久。胰岛素和 IGF-I 的作用依赖于正在进行的翻译和转录。这些结果表明，TSH 和胰岛素/IGF-I 可能通过两种不同的机制降低 c-erbAα1 和 α2 mRNA 水平，而不改变 FRTL-5 细胞中的 c-erbAβ1 mRNA 水平。
Regulation of multiple c-erbAgene expression was studied in rat thyroid FRTL-5 cells. Two species oferbAα (α1 and α2) mRNA and one species oferbAβ (β1) mRNA were identified by Northern blot analysis. Withdrawal of thyrotropin (TSH), insulin and serum from the complete medium resulted in an increase in α1 and α2erbAmRNA levels without altering the level oferbAβ1 mRNA. Readdition of TSH,N6,2′-O-dibutyryl cAMP or forskolin caused a transient reduction of α1 and α2 mRNA levels (75–90%) at 3–12 h. The α1 and α2 mRNA levels were restored at 24 h. The TSH action was dose-dependent showing the half-maximal effect at around 10−9M. Readdition of TSH did not show any effect on β1 mRNA level. The action of TSH was not dependent on ongoing protein synthesis but required ongoing transcription. Inhibitors of thyroid hormone biosynthesis, propylthiouracil and methylmercaptoimidazole, did not show any effect on TSH action. Readdition of insulin or insulin-like growth factor I (IGF-I) caused a dose-dependent reduction of α1 and α2 mRNA levels without any effect on β1 mRNA level. Their action was slower than TSH and persistent. The actions of insulin and IGF-I were dependent on both ongoing translation and transcription. These results indicate that TSH and insulin/IGF-I reduce levels of c-erbAα1 and α2 mRNA possibly by two distinct mechanisms without altering c-erbAβ1 mRNA level in FRTL-5 cells.