Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14
Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14
复制标题
DOI：
10.1128/aem.62.11.4081-4085.1996
复制
发表时间：
1996-11-01
影响因子：
4.4
通讯作者：
Shoda, M
中科院分区：
文献类型：
--
作者：
Asaka, O;Shoda, M
枯草芽孢杆菌RB14在体外对几种植物病原菌具有抗菌活性，其产生的抗菌素为伊图灵A和表面蛋白，通过盆栽试验研究了其对番茄立枯病的抑制能力。为了便于从土壤中恢复，利用枯草芽孢杆菌(B.subtilis)的自然链霉素抗性突变体RB14-C，在土壤中接种RB14-C的发酵液、细胞悬浮液或无细胞发酵液，抑制立枯病的发生，从接种RB14-C的土壤中回收伊图灵A和表面素，证实了RB14-C在土壤中产生这两种抗生素，并将从RB14克隆的产生这两种抗生素所需的基因lpa-14在RB14-C中进行了突变，构建了R Delta 1的抗病能力较低的突变株R Delta 1但R Delta 1(PC115)的转化子R Delta 1(PC115)恢复了抑制性，这些结果表明，RB14产生的抗生素iturin A和表面素在抑制水稻立枯病中起主要作用。在试验期间，RB14-C、R Delta 1和R Delta 1(PC115)在土壤中持续存在，并从土壤中被回收，主要以孢子的形式存在。
Bacillus subtilis RB14, which showed antibiotic activities against several phytopathogens in vitro by producing the antibiotics iturin A and surfactin, was subjected to a pot test to investigate its ability to suppress damping-off of tomato seedlings caused by Rhizoctonia solani. To facilitate recovery from soil, B. subtilis RB14-C, a spontaneous streptomycin-resistant mutant of RB14, was used, Damping-off was suppressed when the culture broth, cell suspension, or cell-free culture broth of RB14-C was inoculated into soil, Iturin A and surfactin were recovered from the soils inoculated with the cell suspension of RB14-C, confirming that RB14-C produced them in soil, The gene lpa-14, which was cloned from RB14 and required for the production of both antibiotics, was mutated in RB14-C, and a mutant, R Delta 1, was constructed, The level of disease suppressibility of R Delta 1 was low, but R Delta 1(pC115), a transformant of R Delta 1 with the plasmid pC115 carrying lpa-14, was restored in suppressibility, These results show that the antibiotics iturin A and surfactin produced by RB14 play a major role in the suppression of damping-off caused by R. solani. RB14-C, R Delta 1, and R Delta 1(pC115) persisted in soil during the experimental period and were recovered from the soil, mostly as spores.