VISUALIZATION OF PEPTIDE-SPECIFIC T-CELL IMMUNITY AND PERIPHERAL TOLERANCE INDUCTION IN-VIVO
VISUALIZATION OF PEPTIDE-SPECIFIC T-CELL IMMUNITY AND PERIPHERAL TOLERANCE INDUCTION IN-VIVO
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DOI：
10.1016/1074-7613(94)90084-1
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发表时间：
1994-07-01
期刊：
影响因子：
32.4
通讯作者：
JENKINS, MK
中科院分区：
文献类型：
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作者：
KEARNEY, ER;PAPE, KA;JENKINS, MK
采用过继转移系统在体内对少量T细胞受体（TCR）转基因T细胞的行为进行了物理监测。在皮下注射佐剂中的抗原后，抗原特异性细胞首先在引流淋巴结的副皮质区聚集，在那里增殖数天，然后进入淋巴结滤泡，在滤泡中它们占T细胞的大部分。然后它们从引流淋巴结缓慢消失，剩余的细胞在体外对抗原刺激高度敏感。相反，当抗原被引入血液时，抗原特异性细胞迅速在所有淋巴结的副皮质区聚集，在那里短暂增殖，但从不进入滤泡。大多数细胞随后迅速消失，留下一群对抗原刺激反应低下的细胞。这些结果为经典的发现提供了物理基础，即抗原特异性记忆和耐受可受抗原给予形式的影响。
An adoptive transfer system was used to monitor physically the behavior of a trace population of TCR transgenic T cells in vivo. After subcutaneous injection of antigen in adjuvant, the antigen-specific cells accumulated first in the paracortical region of the draining lymph nodes, proliferated there for several days, and then moved into lymph node follicles, where they accounted for most of the T cells. They then disappeared slowly from the draining nodes, and the remaining cells were hypersensitive to antigenic stimulation in vitro. In contrast, when the antigen was introduced into the blood, the antigen-specific cells rapidly accumulated in the paracortical regions of all lymph nodes, proliferated there for a short time, but never entered follicles. Most of the cells then rapidly disappeared, leaving behind a population that was hyporesponsive to antigenic stimulation. These results provide a physical basis for the classical finding that antigen-specific memory and tolerance can be influenced by the form of antigen administration.