The microbiome of otitis media with effusion
The microbiome of otitis media with effusion
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DOI：
10.1002/lary.26128
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发表时间：
2016-12-01
期刊：
影响因子：
2.6
通讯作者：
Psaltis, Alkis James
中科院分区：
文献类型：
--
作者：
Chan, Chun Ling;Wabnitz, David;Psaltis, Alkis James
目的/假设在分泌性中耳炎的发病机制中，腺样体垫被认为是细菌的储存库。本研究旨在描述儿童中耳微生物群与分泌性中耳炎，并建立是否存在相关性中耳和腺样体microbiota.Study DesignProspective，controlled study.MethodsMiddle ear aspirates adenoid pad拭子收集了23名儿童进行通气管插入。无耳部疾病患者的腺样体拭子作为对照。样本进行了分析，使用16 S rRNA测序Illumina MiSeq platform.ResultsThirty-five中耳样本收集。中耳渗出液微生物群主要由耳异球菌（23%平均相对丰度）、嗜血杆菌（22%）、莫拉氏菌（5%）和链球菌（5%）组成。异球菌与嗜血杆菌呈负相关（P = 0.049），并且在单侧积液中相对丰度更高（P = 0.004）。来自同一患者的双侧渗出液的微生物群相似（P <0.001）。然而，渗出性中耳炎的微生物群被发现与腺样体的微生物群不同（P = 0.01），而分泌性中耳炎患者和对照组患者的腺样体微生物群相似，（P > 0.05）（Permutational Multivariate Analysis of the Variance）结论腺样体和中耳局部微生物群的差异质疑了腺样体垫是重要储库的理论。中耳炎患儿的中耳炎。中耳炎的累积相对丰度最高，特别是在单侧积液中，并且与嗜血杆菌的相对丰度呈负相关。证据水平NA喉镜，126：2844-2851，2016
Objectives/HypothesisThe adenoid pad has been considered a reservoir for bacteria in the pathogenesis of otitis media with effusion. This study aimed to characterize the middle ear microbiota in children with otitis media with effusion and establish whether a correlation exists between the middle ear and adenoid microbiota.Study DesignProspective, controlled study.MethodsMiddle ear aspirates adenoid pad swabs were collected from 23 children undergoing ventilation tube insertion. Adenoid swabs from patients without ear disease were controls. Samples were analyzed using 16S rRNA sequencing on the Illumina MiSeq platform.ResultsThirty-five middle ear samples were collected. The middle ear effusion microbiota was dominated by Alloiococcus otitidis (23% mean relative abundance), Haemophilus (22%), Moraxella (5%), and Streptococcus (5%). Alloiococcus shared an inverse correlation with Haemophilus (P = .049) and was found in greater relative abundance in unilateral effusion (P = .004). The microbiota of bilateral effusions from the same patient were similar (P < .001). However, the otitis media with effusion microbiota were found to be dissimilar to that of the adenoid (P = .01), whereas the adenoid microbiota of otitis media with effusion and control patients were similar (P > .05) (permutational multivariate analysis of the variance).ConclusionsDissimilarities between the local microbiota of the adenoid and the middle ear question the theory that the adenoid pad is a significant reservoir to the middle ear in children with otitis media with effusion. A otitidis had the greatest cumulative relative abundance, particularly in unilateral effusions, and shares an inverse correlation with the relative abundance of Haemophilus.Level of EvidenceNA Laryngoscope, 126:2844-2851, 2016