Gene Expression Pathways of High Grade Localized Prostate Cancer
Gene Expression Pathways of High Grade Localized Prostate Cancer
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DOI：
10.1002/pros.21373
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发表时间：
2011-10-01
期刊：
影响因子：
2.8
通讯作者：
Schaeffer, Edward M.
中科院分区：
文献类型：
--
作者：
Ross, Ashley E.;Marchionni, Luigi;Schaeffer, Edward M.
背景。尽管在筛查和局部治疗方面取得了进展，前列腺癌仍然是美国男性癌症相关死亡的第二大常见原因，那些患有高级别疾病的人死于前列腺癌的风险最高。在这里，我们确定了区分高级别和低级别病理局限性前列腺癌的基因和细胞途径。采用激光捕获显微解剖方法，从局限性病变男性低分级（Gleason 3 + 3 = 6）或高分级（Gleason 4 + 4 = 8）肿瘤中分离癌细胞。对18,344个独特的注释基因进行了表达谱分析，并使用R/Bioconductor项目的软件包分析数据，以确定差异基因表达并进行基因集富集分析。检索公开可用的表达数据，并以相同的方式和跨平台荟萃分析分别进行分析。670个基因在Gleason sum 6和8肿瘤中差异表达，错误发现率为
BACKGROUND. Despite advances in screening and local therapy, prostate cancer remains the second most common cause of cancer related death among American men, with those having high grade disease being at highest risk for prostate cancer mortality. Here we identify the genes and cellular pathways that distinguish high grade from low grade pathologically localized prostate cancer.METHODS. Cancer cells from low grade (Gleason 3 + 3 = 6) or high grade (4 + 4 = 8) tumors of men with localized disease were isolated by laser capture micro-dissection. Expression profiling was conducted across 18,344 unique annotated genes and data were analyzed using packages from the R/Bioconductor project to determine differential gene expression and perform gene set enrichment analysis. Publically available expression data was retrieved and analyzed individually in the same manner and in cross platform meta-analyses.RESULTS. Six hundred seventy genes were differentially expressed between Gleason sum 6 and 8 tumors with a false discovery rate of